Archives of Pediatric Infectious Diseases

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Estimation of Group B Streptococcus Colonization in High-Risk Neonates by PCR and Standard Culture

Fariba Shirvani 1 , * , Nader Shahrochi 2 , Mitra Radfar 3 and Davood Lakestani 4
Authors Information
1 Pediatric Infections Research Center, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
2 Department of Biotechnology, Pasteur Institute of Iran, Tehran, IR Iran
3 Neonatal Intensive Care Unit, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
4 Pediatric Ward, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
Article information
  • Archives of Pediatric Infectious Diseases: October 01, 2014, 2 (4); e17345
  • Published Online: October 18, 2014
  • Article Type: Research Article
  • Received: January 2, 2014
  • Revised: January 31, 2014
  • Accepted: March 17, 2014
  • DOI: 10.5812/pedinfect.17345

To Cite: Shirvani F, Shahrochi N, Radfar M, Lakestani D. Estimation of Group B Streptococcus Colonization in High-Risk Neonates by PCR and Standard Culture, Arch Pediatr Infect Dis. 2014 ; 2(4):e17345. doi: 10.5812/pedinfect.17345.

Abstract
Copyright © 2014, Pediartric Infections Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Schuchat A. Group B streptococcus. Lancet. 1999; 353(9146): 51-6[DOI][PubMed]
  • 2. Feigin RD, Demmaler-Harrison GJ, Cherry JD, Kaplan SL. Textbook of pediatric infectious diseases. 2009;
  • 3. Javanmanesh F, Eshraghi N. Prevalence of positive recto-vaginal culture for Group B streptococcus in pregnant women at 35-37 weeks of gestation. Med J Islam Repub Iran. 2013; 27(1): 7-11[PubMed]
  • 4. Bakhtiari R, Dallal MS, Mehrabadi J, Heidarzadeh S, Pourmand M. Evaluation of culture and PCR methods for diagnosis of group B streptococcus carriage in Iranian pregnant women. Iran J Public Health. 2012; 41(3): 65-70[PubMed]
  • 5. Shirvani F, Momenzadeh A, Aslani MM, Shahrochi N, Torabi SJ, Khalegi R. Prevalence of vaginal colonization of group B streptococcus in Tehran,s women and its relation to colonization in newborns. Pejouhandeh J. 1998; 2(4): 121-5
  • 6. Fatemi F, Pakzad P, Zeraati H, Talebi S, Asgari S, Akhondi MM, et al. Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor. Iran J Basic Med Sci. 2010; 13(4): 183-8
  • 7. Yu HW, Lin HC, Yang PH, Hsu CH, Hsieh WS, Tsao LY, et al. Group B streptococcal infection in Taiwan: maternal colonization and neonatal infection. Pediatr Neonatol. 2011; 52(4): 190-5[DOI][PubMed]
  • 8. Barcaite E, Bartusevicius A, Tameliene R, Maleckiene L, Vitkauskiene A, Nadisauskiene R. Group B streptococcus and Escherichia coli colonization in pregnant women and neonates in Lithuania. Int J Gynaecol Obstet. 2012; 117(1): 69-73[DOI][PubMed]
  • 9. Yang MJ, Sun PL, Wen KC, Chao KC, Chang WH, Chen CY, et al. Prevalence of maternal group B streptococcus colonization and vertical transmission in low-risk women in a single institute. J Chin Med Assoc. 2012; 75(1): 25-8[DOI][PubMed]
  • 10. Nourian M, Allaii F, Heidari A. Comparison of the effect of alcohol 70% versus dry cord care on cord bacterial colonization and cord separation time among newborns. Pak J Med Sci. 2009; 25(1): 103-7
  • 11. Khosravi N, Noorbakhsh S, Tabatabaei A, Ghavami Y. Prevalence of streptococcus group B in tracheal tube secretions o fneonates with respiratory distress: a brief report. Tehran Univ Med J. 2013; 70(11): 729-34
  • 12. Schrag S, Gorwitz R, Fultz-Butts K, Schuchat A. Prevention of perinatal group B streptococcal disease. MMWR Recomm Rep. 2002; 51(11): 1-22
  • 13. Convert M, Martinetti Lucchini G, Dolina M, Piffaretti JC. Comparison of LightCycler PCR and culture for detection of group B streptococci from vaginal swabs. Clin Microbiol Infect. 2005; 11(12): 1022-6[DOI][PubMed]
  • 14. Lee DY, Shannon K, Beaudette LA. Detection of bacterial pathogens in municipal wastewater using an oligonucleotide microarray and real-time quantitative PCR. J Microbiol Methods. 2006; 65(3): 453-67[DOI][PubMed]
  • 15. Prevention of early onset neonatal group B streptococcal disease Guideline. 2012;
  • 16. Law MR, Palomaki G, Alfirevic Z, Gilbert R, Heath P, McCartney C, et al. The prevention of neonatal group B streptococcal disease: a report by a working group of the Medical Screening Society. J Med Screen. 2005; 12(2): 60-8[DOI][PubMed]
  • 17. Schrag SJ, Zywicki S, Farley MM, Reingold AL, Harrison LH, Lefkowitz LB, et al. Group B streptococcal disease in the era of intrapartum antibiotic prophylaxis. N Engl J Med. 2000; 342(1): 15-20[DOI][PubMed]
  • 18. Heath PT, Schuchat A. Perinatal group B streptococcal disease. Best Pract Res Clin Obstet Gynaecol. 2007; 21(3): 411-24[DOI][PubMed]
  • 19. Pettersson K. Perinatal infection with Group B streptococci. Semin Fetal Neonatal Med. 2007; 12(3): 193-7[DOI][PubMed]
  • 20. Cantoni L, Ronfani L, Da Riol R, Demarini S, Perinatal Study Group of the Region Friuli-Venezia G. Physical examination instead of laboratory tests for most infants born to mothers colonized with group B Streptococcus: support for the Centers for Disease Control and Prevention's 2010 recommendations. J Pediatr. 2013; 163(2): 568-73[DOI][PubMed]
  • 21. Bergseng H, Bevanger L, Rygg M, Bergh K. Real-time PCR targeting the sip gene for detection of group B Streptococcus colonization in pregnant women at delivery. J Med Microbiol. 2007; 56: 223-8[DOI][PubMed]
  • 22. Davies HD, Miller MA, Faro S, Gregson D, Kehl SC, Jordan JA. Multicenter study of a rapid molecular-based assay for the diagnosis of group B Streptococcus colonization in pregnant women. Clin Infect Dis. 2004; 39(8): 1129-35[DOI][PubMed]
  • 23. Gavino M, Wang E. A comparison of a new rapid real-time polymerase chain reaction system to traditional culture in determining group B streptococcus colonization. Am J Obstet Gynecol. 2007; 197(4): 3880-4[DOI][PubMed]
  • 24. Goodrich JS, Miller MB. Comparison of culture and 2 real-time polymerase chain reaction assays to detect group B Streptococcus during antepartum screening. Diagn Microbiol Infect Dis. 2007; 59(1): 17-22[DOI][PubMed]
  • 25. Rallu F, Barriga P, Scrivo C, Martel-Laferriere V, Laferriere C. Sensitivities of antigen detection and PCR assays greatly increased compared to that of the standard culture method for screening for group B streptococcus carriage in pregnant women. J Clin Microbiol. 2006; 44(3): 725-8[DOI][PubMed]
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