Archives of Pediatric Infectious Diseases

Published by: Kowsar

Molecular Detection of blaVEB-1 Beta-Lactamase Encoding Gene Among Extended Spectrum B-Lactamase Positive Wound Isolates of Pseudomonas aeruginosa

Elham Davodian 1 , * , Nourkhoda Sadeghifard 2 , Abdolmajid Ghasemian 3 and Samileh Noorbakhsh 4
Authors Information
1 Department of Microbiology, School of Paramedical Sciences, Ilam University of Medical Sciences, Ilam, IR Iran
2 Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran
3 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
4 Research Center of Pediatric Infectious Diseases, Iran University of Medical Sciences, Tehran, IR Iran
Article information
  • Archives of Pediatric Infectious Diseases: October 01, 2015, 3 (4); e26362
  • Published Online: October 12, 2015
  • Article Type: Research Article
  • Received: December 23, 2014
  • Revised: June 24, 2015
  • Accepted: August 9, 2015
  • DOI: 10.5812/pedinfect.26362

To Cite: Davodian E, Sadeghifard N, Ghasemian A, Noorbakhsh S. Molecular Detection of blaVEB-1 Beta-Lactamase Encoding Gene Among Extended Spectrum B-Lactamase Positive Wound Isolates of Pseudomonas aeruginosa, Arch Pediatr Infect Dis. 2015 ; 3(4):e26362. doi: 10.5812/pedinfect.26362.

Copyright © 2015, Pediartric Infections Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Basak S, Khodke M, Bose S, Mallick SK. Inducible Amp C beta-lactamase producing Pseudomonas aeruginosa isolated in a rural hospital of central India. J Clin Diagn Res. 2009; 3: 19-7
  • 2. Kohlenberg A, Weitzel-Kage D, van der Linden P, Sohr D, Vogeler S, Kola A, et al. Outbreak of carbapenem-resistant Pseudomonas aeruginosa infection in a surgical intensive care unit. J Hosp Infect. 2010; 74(4): 350-7[DOI][PubMed]
  • 3. El Amin N, Giske CG, Jalal S, Keijser B, Kronvall G, Wretlind B. Carbapenem resistance mechanisms in Pseudomonas aeruginosa: alterations of porin OprD and efflux proteins do not fully explain resistance patterns observed in clinical isolates. APMIS. 2005; 113(3): 187-96[DOI][PubMed]
  • 4. Gupta V, Garg R, Garg S, Chander J, Attri AK. Coexistence of Extended Spectrum Beta-Lactamases, AmpC Beta-Lactamases and Metallo-Beta-Lactamases in Acinetobacter baumannii from burns patients: a report from a tertiary care centre of India. Ann Burns Fire Disasters. 2013; 26(4): 189-92[PubMed]
  • 5. Qi Y, Wei Z, Ji S, Du X, Shen P, Yu Y. ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China. J Antimicrob Chemother. 2011; 66(2): 307-12[DOI][PubMed]
  • 6. Strateva T, Yordanov D. Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol. 2009; 58: 1133-48[DOI][PubMed]
  • 7. Mansour W, Dahmen S, Poirel L, Charfi K, Bettaieb D, Boujaafar N, et al. Emergence of SHV-2a extended-spectrum beta-lactamases in clinical isolates of Pseudomonas aeruginosa in a university hospital in Tunisia. Microb Drug Resist. 2009; 15(4): 295-301[DOI][PubMed]
  • 8. Bonnet R. Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother. 2004; 48(1): 1-14[PubMed]
  • 9. Luzzaro F, Mezzatesta M, Mugnaioli C, Perilli M, Stefani S, Amicosante G, et al. Trends in production of extended-spectrum beta-lactamases among enterobacteria of medical interest: report of the second Italian nationwide survey. J Clin Microbiol. 2006; 44(5): 1659-64[DOI][PubMed]
  • 10. Shahcheraghi F, Nikbin VS, Feizabadi MM. Prevalence of ESBLs genes among multidrug-resistant isolates of Pseudomonas aeruginosa isolated from patients in Tehran. Microb Drug Resist. 2009; 15(1): 37-9[DOI][PubMed]
  • 11. Papp-Wallace KM, Endimiani A, Taracila MA, Bonomo RA. Carbapenems: past, present, and future. Antimicrob Agents Chemother. 2011; 55(11): 4943-60[DOI][PubMed]
  • 12. Poirel L, Menuteau O, Agoli N, Cattoen C, Nordmann P. Outbreak of extended-spectrum beta-lactamase VEB-1-producing isolates of Acinetobacter baumannii in a French hospital. J Clin Microbiol. 2003; 41(8): 3542-7[PubMed]
  • 13. Ghasemian A, Peerayeh SN, Bakhshi B, Mirzaee M. Detection of accessory gene regulator groups genes and cassette chromosome mec types among Staphylococcus aureus isolated from intensive care unit patients. Asian Pacific J Trop Dis. 2015; 5(2): 153-7[DOI]
  • 14. Ghasemian A, Najar Peerayeh S, Bakhshi B, Mirzaee M. Accessory Gene Regulator Specificity Groups Among Staphylococcus aureus Isolated From Hospitalized Children. Arch Pediatr Infect Dis. 2014; 2(2)[DOI]
  • 15. Mesaros N, Nordmann P, Plesiat P, Roussel-Delvallez M, Van Eldere J, Glupczynski Y, et al. Pseudomonas aeruginosa: resistance and therapeutic options at the turn of the new millennium. Clin Microbiol Infect. 2007; 13(6): 560-78[DOI][PubMed]
  • 16. Hosseini-Mazinani SM, Eftekhar F, Milani M, Ghandili S. Characterization of beta-lactamases from urinary isolates of Escherichia coli in Tehran. Iran Biomed J. 2007; 11(2): 95-9[PubMed]
  • 17. Garrec H, Drieux-Rouzet L, Golmard JL, Jarlier V, Robert J. Comparison of nine phenotypic methods for detection of extended-spectrum beta-lactamase production by Enterobacteriaceae. J Clin Microbiol. 2011; 49(3): 1048-57[DOI][PubMed]
  • 18. Wolter DJ, Schmidtke AJ, Hanson ND, Lister PD. Increased expression of ampC in Pseudomonas aeruginosa mutants selected with ciprofloxacin. Antimicrob Agents Chemother. 2007; 51(8): 2997-3000[DOI][PubMed]
  • 19. Salimi F, Eftekhar F. Coexistence of AmpC and Extended-Spectrum β-lactamases in Metallo-β-Lactamase Producing Pseudomonas aeruginosa Burn Isolates in Tehran. Jundishapure J Microbiol. 2013; 6(8)[DOI]
  • 20. Shacheraghi F, Shakibaie MR, Noveiri H. Molecular Identification of ESBL genes bla<sub>GES-1</sub>, bla<sub>VEB-1</sub>, bla<sub>CTX-M</sub> bla<sub>OXA-1</sub>, bla<sub>OXA-4</sub>, bla<sub>OXA-10</sub> and bla<sub>PER-1</sub> in Pseudomonas aeruginosa strains isolated from burn patients by PCR, RFLP and sequencing techniques. Int J Biol life Sci. 2010; 3(6): 138-42
  • 21. Mirsalehian A, Feizabadi M, Nakhjavani FA, Jabalameli F, Goli H, Kalantari N. Detection of VEB-1, OXA-10 and PER-1 genotypes in extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients. Burns. 2010; 36(1): 70-4[DOI][PubMed]
  • 22. Irajian G, Jazayeri Moghadas A. Frequency of extended-spectrum beta lactamase positive and multidrug resistance pattern in Gram-negative urinary isolates, Semnan, Iran. Jundishapur J Microbiol. 2010; 3(3): 107-13
  • 23. Aminzadeh Z, Yadegarynia D, Fatemi A, Azad Armaki S, Aslanbeygi B. Prevalence and Antimicrobial Susceptibility Pattern of Extended Spectrum Beta Lactamase (ESBL) and non-ESBL Producing Enteric Gram-Negative Bacteria and Activity of Nitrofurantoin in the era of ESBL. Jundishapur J Microbiol. 2013; 6(7)[DOI]
  • 24. Khosravi AD, Hoveizavi H, Mehdinejad M. Prevalence of Klebsiella pneumoniae Encoding Genes for Ctx-M-1, Tem-1 and Shv-1 Extended-Spectrum Beta Lactamases (ESBL) Enzymes in Clinical Specimens. Jundishapur J Microbiol. 2013; 6(10)[DOI]
  • 25. Fazeli H, Dolatabadi RK, Taraghian A, Nasr Isfahani B, Moghim S, Norouzi M. Carbapenem Resistance Pattern of Multiple Drug-Resistantand Extended-Spectrum Beta-Lactamase-Positive Klebsiella pneumonia in Isfahan. Int J Enteric Pathog. 2014; 2(4)
  • 26. Kapur A, John AS. Prevalence of Extended-Spectrum Beta-Lactamase-Producing Pathogens From Urinary Tract Infected Samples and Their Sensitivity Pattern Against. Int J Infect. 2015; 2(1)
  • 27. Hill D, Rose B, Pajkos A, Robinson M, Bye P, Bell S, et al. Antibiotic susceptabilities of Pseudomonas aeruginosa isolates derived from patients with cystic fibrosis under aerobic, anaerobic, and biofilm conditions. J Clin Microbiol. 2005; 43(10): 5085-90[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments