Archives of Pediatric Infectious Diseases

Published by: Kowsar

Distribution of Virulence Genes, Enterotoxin and Biofilm Formation Among Enteroaggregative Escherichia Coli (EAEC) Strains Isolated From Stools of Children With Diarrhea in South East Iran

Razieh Nezarieh 1 , Mohammad Reza Shakibaie 1 , 2 , * , Hossein Hosseini Nave 1 , Amin Norouzi 1 , Gholamabas Salajegheh 3 and Mehdi Hayatbakhsh 4
Authors Information
1 Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, IR Iran
2 Research Center for Infectious Diseases and Tropical Medicine, Kerman University of Medical Sciences, Kerman, IR Iran
3 Division of Internal Medicine, Department of Infectious Diseases, Afzalipour Hospital, Kerman, IR Iran
4 Gastroenterology and Hepatology Research Center, Kerman University of Medical Sciences, Kerman, IR Iran
Article information
  • Archives of Pediatric Infectious Diseases: July 01, 2015, 3 (3); e29745
  • Published Online: July 1, 2015
  • Article Type: Research Article
  • Received: May 5, 2015
  • Revised: May 12, 2015
  • Accepted: May 15, 2015
  • DOI: 10.5812/pedinfect.29745v2

To Cite: Nezarieh R, Shakibaie M R, Hosseini Nave H, Norouzi A, Salajegheh G, et al. Distribution of Virulence Genes, Enterotoxin and Biofilm Formation Among Enteroaggregative Escherichia Coli (EAEC) Strains Isolated From Stools of Children With Diarrhea in South East Iran, Arch Pediatr Infect Dis. 2015 ; 3(3):e29745. doi: 10.5812/pedinfect.29745v2.

Abstract
Copyright © 2015, Pediartric Infections Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
Acknowledgements
Footnote
References
  • 1. O'Reilly CE, Jaron P, Ochieng B, Nyaguara A, Tate JE, Parsons MB, et al. Risk factors for death among children less than 5 years old hospitalized with diarrhea in rural western Kenya, 2005-2007: a cohort study. PLoS Med. 2012; 9(7)[DOI][PubMed]
  • 2. Lanata CF, Fischer-Walker CL, Olascoaga AC, Torres CX, Aryee MJ, Black RE, et al. Global causes of diarrheal disease mortality in children <5 years of age: a systematic review. PLoS One. 2013; 8(9)[DOI][PubMed]
  • 3. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol. 2004; 2(2): 123-40[DOI][PubMed]
  • 4. Weintraub A. Enteroaggregative Escherichia coli: epidemiology, virulence and detection. J Med Microbiol. 2007; 56: 4-8[DOI][PubMed]
  • 5. Estrada-Garcia T, Navarro-Garcia F. Enteroaggregative Escherichia coli pathotype: a genetically heterogeneous emerging foodborne enteropathogen. FEMS Immunol Med Microbiol. 2012; 66(3): 281-98[DOI][PubMed]
  • 6. Estrada-García T, Cerna JF, Paheco-Gil L, Velázquez RF, Ochoa TJ, Torres J, et al. Drug-resistant DiarrheogenicEscherichia coli, Mexico. Emerg Infect Dis. 2005; 11(8): 1306-8[DOI]
  • 7. Nataro JP, Kaper JB, Robins-Browne R, Prado V, Vial P, Levine MM. Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatr Infect Dis J. 1987; 6(9): 829-31[PubMed]
  • 8. Scavia G, Staffolani M, Fisichella S, Striano G, Colletta S, Ferri G, et al. Enteroaggregative Escherichia coli associated with a foodborne outbreak of gastroenteritis. J Med Microbiol. 2008; 57: 1141-6[DOI][PubMed]
  • 9. Harrington SM, Dudley EG, Nataro JP. Pathogenesis of enteroaggregative Escherichia coli infection. FEMS Microbiol Lett. 2006; 254(1): 12-8[DOI][PubMed]
  • 10. Beutin L, Hammerl JA, Reetz J, Strauch E. Shiga toxin-producing Escherichia coli strains from cattle as a source of the Stx2a bacteriophages present in enteroaggregative Escherichia coli O104:H4 strains. Int J Med Microbiol. 2013; 303(8): 595-602[DOI][PubMed]
  • 11. Morabito S, Karch H, Mariani-Kurkdjian P, Schmidt H, Minelli F, Bingen E, et al. Enteroaggregative, Shiga toxin-producing Escherichia coli O111:H2 associated with an outbreak of hemolytic-uremic syndrome. J Clin Microbiol. 1998; 36(3): 840-2[PubMed]
  • 12. Hebbelstrup Jensen B, Olsen KE, Struve C, Krogfelt KA, Petersen AM. Epidemiology and clinical manifestations of enteroaggregative Escherichia coli. Clin Microbiol Rev. 2014; 27(3): 614-30[DOI][PubMed]
  • 13. Cerna JF, Nataro JP, Estrada-Garcia T. Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains. J Clin Microbiol. 2003; 41(5): 2138-40[PubMed]
  • 14. Kahali S, Sarkar B, Rajendran K, Khanam J, Yamasaki S, Nandy RK, et al. Virulence Characteristics and Molecular Epidemiology of Enteroaggregative Escherichia coli Isolates from Hospitalized Diarrheal Patients in Kolkata, India. J Clin Microbiol. 2004; 42(9): 4111-20[DOI]
  • 15. Vila J, Vargas M, Henderson IR, Gascon J, Nataro JP. Enteroaggregative escherichia coli virulence factors in traveler's diarrhea strains. J Infect Dis. 2000; 182(6): 1780-3[DOI][PubMed]
  • 16. Navarro-Garcia F, Elias WP. Autotransporters and virulence of enteroaggregative E. coli. Gut Microbes. 2011; 2(1): 13-24[DOI][PubMed]
  • 17. Restieri C, Garriss G, Locas MC, Dozois CM. Autotransporter-encoding sequences are phylogenetically distributed among Escherichia coli clinical isolates and reference strains. Appl Environ Microbiol. 2007; 73(5): 1553-62[DOI][PubMed]
  • 18. Boisen N, Ruiz-Perez F, Scheutz F, Krogfelt KA, Nataro JP. Short report: high prevalence of serine protease autotransporter cytotoxins among strains of enteroaggregative Escherichia coli. Am J Trop Med Hyg. 2009; 80(2): 294-301[PubMed]
  • 19. Aslani MM, Alikhani MY, Zavari A, Yousefi R, Zamani AR. Characterization of enteroaggregative Escherichia coli (EAEC) clinical isolates and their antibiotic resistance pattern. Int J Infect Dis. 2011; 15(2)-9[DOI][PubMed]
  • 20. Abbasi P, Kargar M, Doosti A, Mardaneh J, Ghorbani-Dalini S, Anvarinejad M, et al. Enteroaggregative Escherichia Coli (EAEC) in South of Iran. Int J Pediatr. 2014; 2(2): 32
  • 21. Bouzari S, Jafari A, Azizi A, Oloomi M, Nataro JP. Short report: characterization of enteroaggregative Escherichia coli isolates from Iranian children. Am J Trop Med Hyg. 2001; 65(1): 13-4[PubMed]
  • 22. Shakibaie M, Forootanfar H, Golkari Y, Mohammadi-Khorsand T, Shakibaie MR. Anti-biofilm activity of biogenic selenium nanoparticles and selenium dioxide against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis. J Trace Elem Med Biol. 2015; 29: 235-41[DOI][PubMed]
  • 23. Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. 2007; 115(8): 891-9[DOI][PubMed]
  • 24. Dallman TJ, Chattaway MA, Cowley LA, Doumith M, Tewolde R, Wooldridge DJ, et al. An investigation of the diversity of strains of enteroaggregative Escherichia coli isolated from cases associated with a large multi-pathogen foodborne outbreak in the UK. PLoS One. 2014; 9(5)[DOI][PubMed]
  • 25. Eslava C, Navarro-Garcia F, Czeczulin JR, Henderson IR, Cravioto A, Nataro JP. Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli. Infect Immun. 1998; 66(7): 3155-63[PubMed]
  • 26. Modarresi F, Azizi O, Shakibaie MR, Motamedifar M, Mosadegh E, Mansouri S. Iron limitation enhances acyl homoserine lactone (AHL) production and biofilm formation in clinical isolates of Acinetobacter baumannii. Virulence. 2015; 6(2): 152-61[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments