Archives of Pediatric Infectious Diseases

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Molecular Tests for Detection of Cytomegalovirus Infection, Useful Guidance or Misleading Advertisement?

Abdollah Karimi 1 and Ali Amanati 2 , *
Authors Information
1 Pediatric Infections Research Center, Mofid Children’s Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
2 Professor Alborzi Clinical Microbiology Research Center, Amir Oncology Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran
Article information
  • Archives of Pediatric Infectious Diseases: January 01, 2016, 4 (1); e31817
  • Published Online: January 9, 2016
  • Article Type: Editorial
  • Received: July 25, 2015
  • Accepted: August 9, 2015
  • DOI: 10.5812/pedinfect.31817

To Cite: Karimi A, Amanati A. Molecular Tests for Detection of Cytomegalovirus Infection, Useful Guidance or Misleading Advertisement?, Arch Pediatr Infect Dis. 2016 ; 4(1):e31817. doi: 10.5812/pedinfect.31817.

Copyright © 2016, Pediartric Infections Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
References
  • 1. Raggam RB, Bozic M, Salzer HJ, Hammerschmidt S, Homberg C, Ruzicka K, et al. Rapid quantitation of cytomegalovirus DNA in whole blood by a new molecular assay based on automated sample preparation and real-time PCR. Med Microbiol Immunol. 2010; 199(4): 311-6[DOI][PubMed]
  • 2. Coll O, Benoist G, Ville Y, Weisman LE, Botet F, Anceschi MM, et al. Guidelines on CMV congenital infection. J Perinat Med. 2009; 37(5): 433-45[DOI][PubMed]
  • 3. Kraft CS, Armstrong WS, Caliendo AM. Interpreting quantitative cytomegalovirus DNA testing: understanding the laboratory perspective. Clin Infect Dis. 2012; 54(12): 1793-7[DOI][PubMed]
  • 4. Lübeck PR, Berger A, Doerr HW, Rabenau HF. Can the HCMV-pp65-antigenemia assay completely be replaced by HCMV-PCR in monitoring patients after bone marrow transplantation? Rev Infect. 2010; 1(3): 166-71
  • 5. Roberts TC, Buller RS, Gaudreault-Keener M, Sternhell KE, Garlock K, Singer GG, et al. Effects of storage temperature and time on qualitative and quantitative detection of cytomegalovirus in blood specimens by shell vial culture and PCR. J Clin Microbiol. 1997; 35(9): 2224-8[PubMed]
  • 6. Razonable RR, Brown RA, Wilson J, Groettum C, Kremers W, Espy M, et al. The clinical use of various blood compartments for cytomegalovirus (CMV) DNA quantitation in transplant recipients with CMV disease. Transplantation. 2002; 73(6): 968-73[PubMed]
  • 7. Hayden RT, Yan X, Wick MT, Rodriguez AB, Xiong X, Ginocchio CC, et al. Factors contributing to variability of quantitative viral PCR results in proficiency testing samples: a multivariate analysis. J Clin Microbiol. 2012; 50(2): 337-45[DOI][PubMed]
  • 8. Lee AV, Atkinson C, Manuel RJ, Clark DA. Comparative evaluation of the QIAGEN QIAsymphony(R) SP system and bioMerieux NucliSens easyMAG automated extraction platforms in a clinical virology laboratory. J Clin Virol. 2011; 52(4): 339-43[DOI][PubMed]
  • 9. Pang XL, Fox JD, Fenton JM, Miller GG, Caliendo AM, Preiksaitis JK, et al. Interlaboratory comparison of cytomegalovirus viral load assays. Am J Transplant. 2009; 9(2): 258-68[DOI][PubMed]
  • 10. Mengelle C, Mansuy JM, Da Silva I, Davrinche C, Izopet J. Comparison of 2 highly automated nucleic acid extraction systems for quantitation of human cytomegalovirus in whole blood. Diagn Microbiol Infect Dis. 2011; 69(2): 161-6[DOI][PubMed]
  • 11. Atabani SF, Smith C, Atkinson C, Aldridge RW, Rodriguez-Peralvarez M, Rolando N, et al. Cytomegalovirus replication kinetics in solid organ transplant recipients managed by preemptive therapy. Am J Transplant. 2012; 12(9): 2457-64[DOI][PubMed]
  • 12. Munoz-Cobo B, Solano C, Costa E, Bravo D, Clari MA, Benet I, et al. Dynamics of cytomegalovirus (CMV) plasma DNAemia in initial and recurrent episodes of active CMV infection in the allogeneic stem cell transplantation setting: implications for designing preemptive antiviral therapy strategies. Biol Blood Marrow Transplant. 2011; 17(11): 1602-11[DOI][PubMed]
  • 13. Cardenoso L, Pinsky BA, Lautenschlager I, Aslam S, Cobb B, Vilchez RA, et al. CMV antigenemia and quantitative viral load assessments in hematopoietic stem cell transplant recipients. J Clin Virol. 2013; 56(2): 108-12[DOI][PubMed]
  • 14. Chemaly RF, Yen-Lieberman B, Chapman J, Reilly A, Bekele BN, Gordon SM, et al. Clinical utility of cytomegalovirus viral load in bronchoalveolar lavage in lung transplant recipients. Am J Transplant. 2005; 5(3): 544-8[DOI][PubMed]
  • 15. Fryer JF, Heath AB, Anderson R, Minor PD, Unit B, et al. Collaborative study to evaluate the proposed 1st [first] WHO international standard for human cytomegalovirus (HCMV) for nucleic acid amplification (NAT)-based assays. 2010;
  • 16. Furione M, Rognoni V, Cabano E, Baldanti F. Kinetics of human cytomegalovirus (HCMV) DNAemia in transplanted patients expressed in international units as determined with the Abbott RealTime CMV assay and an in-house assay. J Clin Virol. 2012; 55(4): 317-22[DOI][PubMed]
  • 17. Schnepf N, Scieux C, Resche-Riggon M, Feghoul L, Xhaard A, Gallien S, et al. Fully automated quantification of cytomegalovirus (CMV) in whole blood with the new sensitive Abbott RealTime CMV assay in the era of the CMV international standard. J Clin Microbiol. 2013; 51(7): 2096-102[DOI][PubMed]
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