Archives of Pediatric Infectious Diseases

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Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran

Maryam Koshesh 1 , Shahla Mansouri 1 , Zahra Hashemizadeh 1 and Davood Kalantar-Neyestanaki 1 , *
Authors Information
1 Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran
Article information
  • Archives of Pediatric Infectious Diseases: April 01, 2017, 5 (2); e37968
  • Published Online: August 6, 2016
  • Article Type: Research Article
  • Received: April 5, 2016
  • Revised: June 7, 2016
  • Accepted: June 20, 2016
  • DOI: 10.5812/pedinfect.37968

To Cite: Koshesh M, Mansouri S, Hashemizadeh Z, Kalantar-Neyestanaki D. Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran, Arch Pediatr Infect Dis. 2017 ; 5(2):e37968. doi: 10.5812/pedinfect.37968.

Copyright © 2016, Pediartric Infections Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Pfeifer Y, Cullik A, Witte W. Resistance to cephalosporins and carbapenems in Gram-negative bacterial pathogens. Int J Med Microbiol. 2010; 300(6): 371-9[DOI][PubMed]
  • 2. Mansouri S, Kalantar Neyestanaki D, Shokoohi M, Halimi S, Beigverdi R, Rezagholezadeh F, et al. Characterization of AmpC, CTX-M and MBLs types of beta-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli producing Extended Spectrum beta-lactamases in Kerman, Iran. Jundishapur J Microbiol. 2014; 7(2)[DOI][PubMed]
  • 3. Poole K. Pseudomonas aeruginosa: resistance to the max. Front Microbiol. 2011; 2: 65[DOI][PubMed]
  • 4. Denton M. Enterobacteriaceae. Int J Antimicrob Agents. 2007; 29 Suppl 3[DOI][PubMed]
  • 5. Falagas ME, Karageorgopoulos DE. Extended-spectrum beta-lactamase-producing organisms. J Hosp Infect. 2009; 73(4): 345-54[DOI][PubMed]
  • 6. Sundin DR. Hidden Beta-Lactamases in the Enterobacteriaceae – Dropping the Extra Disks for Detection, Part II. Clin Microbiol Newsl. 2009; 31(7): 47-52[DOI]
  • 7. Soltani J, Poorabbas B, Miri N, Mardaneh J. Health care associated infections, antibiotic resistance and clinical outcome: A surveillance study from Sanandaj, Iran. World J Clin Cases. 2016; 4(3): 63-70[DOI][PubMed]
  • 8. Navidinia M, Peerayeh SN, Fallah F, Bakhshi B. Phylogenetic groups and pathogenicity island markers in Escherichia coli isolated from children. Jundishapur J Microbiol. 2013; 6(10)
  • 9. Aulet de Saab OC, de Castillo MC, de Ruiz Holgado AP, de Nader OM. A comparative study of preservation and storage of Haemophilus influenzae. Mem Inst Oswaldo Cruz. 2001; 96(4): 583-6[PubMed]
  • 10. Martin WJ, Washington II. J. A. . Enterobacteriaceace. Manual of clinical microbiology. 1980; : 195-219
  • 11. Clinical and Laboratory Standards Institute . Performance standards for antimicrobial susceptibility testing; twenty-fourth informational supplement. 2014; 34
  • 12. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012; 18(3): 268-81[DOI][PubMed]
  • 13. Detection of extended-spectrum β-lactamases (ESBLs) in E. coli and Klebsiella species.
  • 14. Poorabbas B, Mardaneh J, Rezaei Z, Kalani M, Pouladfar G, Alami MH, et al. Nosocomial Infections: Multicenter surveillance of antimicrobial resistance profile of Staphylococcus aureus and Gram negative rods isolated from blood and other sterile body fluids in Iran. Iran J Microbiol. 2015; 7(3): 127-35[PubMed]
  • 15. Black JA, Moland ES, Thomson KS. AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases. J Clin Microbiol. 2005; 43(7): 3110-3[DOI][PubMed]
  • 16. Mirsalehian A, Kalantar-Neyestanaki D, Nourijelyani K, Asadollahi K, Taherikalani M, Emaneini M, et al. Detection of AmpC-beta-lactamases producing isolates among carbapenem resistant P. aeruginosa isolated from burn patient. Iran J Microbiol. 2014; 6(5): 306-10[PubMed]
  • 17. Doi Y, Paterson DL. Detection of plasmid-mediated class C beta-lactamases. Int J Infect Dis. 2007; 11(3): 191-7[DOI][PubMed]
  • 18. Pitout JD, Gregson DB, Poirel L, McClure JA, Le P, Church DL. Detection of Pseudomonas aeruginosa producing metallo-beta-lactamases in a large centralized laboratory. J Clin Microbiol. 2005; 43(7): 3129-35[DOI][PubMed]
  • 19. Coudron PE. Inhibitor-based methods for detection of plasmid-mediated AmpC beta-lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis. J Clin Microbiol. 2005; 43(8): 4163-7[DOI][PubMed]
  • 20. Shakibaie MR, Shahcheraghi F, Hashemi A, Adeli NS. Detection of TEM, SHV and PER Type Extended-Spectrum ß-Lactamase Genes among Clinical Strains of Pseudomonas aeruginosa Isolated from Burnt Patients at Shafa-Hospital, Kerman, Iran. Iran J Basic Med Sci. 2008; 11(2): 104-11
  • 21. Neyestanaki DK, Mirsalehian A, Rezagholizadeh F, Jabalameli F, Taherikalani M, Emaneini M. Determination of extended spectrum beta-lactamases, metallo-beta-lactamases and AmpC-beta-lactamases among carbapenem resistant Pseudomonas aeruginosa isolated from burn patients. Burns. 2014; 40(8): 1556-61[DOI][PubMed]
  • 22. Weldhagen GF, Poirel L, Nordmann P. Ambler class A extended-spectrum beta-lactamases in Pseudomonas aeruginosa: novel developments and clinical impact. Antimicrob Agents Chemother. 2003; 47(8): 2385-92[PubMed]
  • 23. Colom K, Perez J, Alonso R, Fernandez-Aranguiz A, Larino E, Cisterna R. Simple and reliable multiplex PCR assay for detection of blaTEM, bla(SHV) and blaOXA-1 genes in Enterobacteriaceae. FEMS Microbiol Lett. 2003; 223(2): 147-51[PubMed]
  • 24. Qi C, Pilla V, Yu JH, Reed K. Changing prevalence of Escherichia coli with CTX-M-type extended-spectrum beta-lactamases in outpatient urinary E. coli between 2003 and 2008. Diagn Microbiol Infect Dis. 2010; 67(1): 87-91[DOI][PubMed]
  • 25. Kahlmeter G, Poulsen HO. Antimicrobial susceptibility of Escherichia coli from community-acquired urinary tract infections in Europe: the ECO.SENS study revisited. Int J Antimicrob Agents. 2012; 39(1): 45-51[DOI][PubMed]
  • 26. Soltan Dallal M, Sabbaghi A, Molla Aghamirzaeie H, Rastegar Lari A, Eshraghian MR, Fallah Mehrabad J, et al. Prevalence of ampc and shv β-lactamases in clinical isolates of escherichia coli from tehran hospitals. Jundishapur J M. 2013; 6(2)[DOI]
  • 27. Zaniani FR, Meshkat Z, Naderi Nasab M, Khaje-Karamadini M, Ghazvini K, Rezaee A, et al. The Prevalence of TEM and SHV Genes among Extended-Spectrum Beta-Lactamases Producing Escherichia Coli and Klebsiella Pneumoniae. Iran J Basic Med Sci. 2012; 15(1): 654-60[PubMed]
  • 28. Fallah F, Karimi A, Goudarzi M, Shiva F, Navidinia M, Jahromi MH, et al. Determination of integron frequency by a polymerase chain reaction-restriction fragment length polymorphism method in multidrug-resistant Escherichia coli, which causes urinary tract infections. Microb Drug Resist. 2012; 18(6): 546-9[DOI][PubMed]
  • 29. Kalantar D, Mansouri S. Emergence of multiple β-lactamases produced by Escherichia coli clinical isolates from hospitalized patient in Kerman, Iran. Jundishapur J Microbiol. 2010; 3(4): 137-45
  • 30. Falagas ME, Rafailidis PI, Matthaiou DK. Resistance to polymyxins: Mechanisms, frequency and treatment options. Drug Resist Updat. 2010; 13(4-5): 132-8[DOI][PubMed]
  • 31. Navidinia M, Peerayeh SN, Fallah F, Bakhshi B, Sajadinia RS. Phylogenetic grouping and pathotypic comparison of urine and fecal Escherichia coli isolates from children with urinary tract infection. Braz J Microbiol. 2014; 45(2): 509-14[PubMed]
  • 32. Larson E. Community factors in the development of antibiotic resistance. Annu Rev Public Health. 2007; 28: 435-47[DOI][PubMed]
  • 33. Mansouri S, Abbasi S. Prevalence of Multiple Drug Resistant Clinical Isolates of Extended-Spectrum Beta-Lactamase Producing Enterobacteriaceae in Southeast Iran. . Iran J Med Sci. 2010; 35(2): 101-8
  • 34. Abdi S, Ranjbar R, Hakemi VM, Jonaidi N, Baghery BO, Baghery BF. Frequency of bla TEM, bla SHV, bla CTX-M,and qnrAAmong Escherichia coli Isolated From Urinary Tract Infection. Arch Clin Infect Dis. 2014; 9(1)
  • 35. Karimi A, Rahbar M, Fallah F, Navidinia M, Malekan MA. Detection of integron elements and gene groups encoding ESBLs and their prevalence in Escherichia coli and Klebsiella isolated from urine samples by PCR method. Afr J Microbiol Res. 2012; 6(8)[DOI]
  • 36. Reuland EA, Halaby T, Hays JP, de Jongh DM, Snetselaar HD, van Keulen M, et al. Plasmid-mediated AmpC: prevalence in community-acquired isolates in Amsterdam, the Netherlands, and risk factors for carriage. PLoS One. 2015; 10(1)[DOI][PubMed]
  • 37. Altoparlak U, Aktas F, Celebi D, Ozkurt Z, Akcay MN. Prevalence of metallo-beta-lactamase among Pseudomonas aeruginosa and Acinetobacter baumannii isolated from burn wounds and in vitro activities of antibiotic combinations against these isolates. Burns. 2005; 31(6): 707-10[DOI][PubMed]
  • 38. Kang CI, Wi YM, Lee MY, Ko KS, Chung DR, Peck KR, et al. Epidemiology and risk factors of community onset infections caused by extended-spectrum beta-lactamase-producing Escherichia coli strains. J Clin Microbiol. 2012; 50(2): 312-7[DOI][PubMed]
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