Archives of Pediatric Infectious Diseases

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Study of Cronobacter sakazakii Strains Isolated from Powdered Milk Infant Formula by Phenotypic and Molecular Methods in Iran

Jalal Mardaneh 1 and Mohammad Mehdi Soltan Dallal 2 , 3 , *
Authors Information
1 Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
2 Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Pathobiology, Division of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Article information
  • Archives of Pediatric Infectious Diseases: January 01, 2017, 5 (1); e38867
  • Published Online: September 6, 2016
  • Article Type: Research Article
  • Received: May 2, 2016
  • Revised: July 25, 2016
  • Accepted: August 14, 2016
  • DOI: 10.5812/pedinfect.38867

To Cite: Mardaneh J, Soltan Dallal M M. Study of Cronobacter sakazakii Strains Isolated from Powdered Milk Infant Formula by Phenotypic and Molecular Methods in Iran, Arch Pediatr Infect Dis. 2017 ; 5(1):e38867. doi: 10.5812/pedinfect.38867.

Copyright © 2016, Pediartric Infections Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Joseph S, Forsythe SJ. Predominance of Cronobacter sakazakii sequence type 4 in neonatal infections. Emerg Infect Dis. 2011; 17(9): 1713-5[DOI][PubMed]
  • 2. Chap J, Jackson P, Siqueira R, Gaspar N, Quintas C, Park J, et al. International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant foods. Int J Food Microbiol. 2009; 136(2): 185-8[DOI][PubMed]
  • 3. Drudy D, Mullane NR, Quinn T, Wall PG, Fanning S. Enterobacter sakazakii: an emerging pathogen in powdered infant formula. Clin Infect Dis. 2006; 42(7): 996-1002[DOI][PubMed]
  • 4. Fiore A, Casale M, Aureli P. Enterobacter sakazakii: epidemiology, clinical presentation, prevention and control. Ann Ist Super Sanita. 2008; 44(3): 275-80[PubMed]
  • 5. Beuchat LR, Kim H, Gurtler JB, Lin LC, Ryu JH, Richards GM. Cronobacter sakazakii in foods and factors affecting its survival, growth, and inactivation. Int J Food Microbiol. 2009; 136(2): 204-13[DOI][PubMed]
  • 6. Baumgartner A, Grand M, Liniger M, Iversen C. Detection and frequency of Cronobacter spp. (Enterobacter sakazakii) in different categories of ready-to-eat foods other than infant formula. Int J Food Microbiol. 2009; 136(2): 189-92[DOI][PubMed]
  • 7. Caubilla-Barron J, Hurrell E, Townsend S, Cheetham P, Loc-Carrillo C, Fayet O, et al. Genotypic and phenotypic analysis of Enterobacter sakazakii strains from an outbreak resulting in fatalities in a neonatal intensive care unit in France. J Clin Microbiol. 2007; 45(12): 3979-85[DOI][PubMed]
  • 8. Dumen E. Cronobacter sakazakii (Enterobacter sakazakii). Only An Infant Problem? Kafkas Univ Vet Fak Derg. 2010; 16: 171-8
  • 9. Muytjens HL, Roelofs-Willemse H, Jaspar GH. Quality of powdered substitutes for breast milk with regard to members of the family Enterobacteriaceae. J Clin Microbiol. 1988; 26(4): 743-6[PubMed]
  • 10. Shaker R, Osaili T, Al-Omary W. Isolation of Enterobacter sakazakii and other Enterobacter sp. from food and food production environments. Food Control. 2007; 18: 1241-5[DOI]
  • 11. Li R, Fei P, Man CX, Lou BB, Niu JT, Feng J, et al. Tea polyphenols inactivate Cronobacter sakazakii isolated from powdered infant formula. J Dairy Sci. 2016; 99(2): 1019-28[DOI][PubMed]
  • 12. Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, et al. Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. Int J Syst Evol Microbiol. 2008; 58: 1442-7[DOI][PubMed]
  • 13. Lee HA, Hong S, Park H, Kim H, Kim O. Cronobacter sakazakii Infection Induced Fatal Clinical Sequels Including Meningitis in Neonatal ICR Mice. Lab Anim Res. 2011; 27(1): 59-62[DOI][PubMed]
  • 14. Feeney A, Kropp KA, O'Connor R, Sleator RD. Cronobacter sakazakii: stress survival and virulence potential in an opportunistic foodborne pathogen. Gut Microbes. 2014; 5(6): 711-8[DOI][PubMed]
  • 15. Iversen C, Forsythe S. Risk profile of Enterobacter sakazakii, an emergent pathogen associated with infant milk formula. Trends Food Sci Technol. 2003; 14: 443-54[DOI]
  • 16. Kalyantanda G, Shumyak L, Archibald LK. Cronobacter Species Contamination of Powdered Infant Formula and the Implications for Neonatal Health. Front Pediatr. 2015; 3: 56[DOI][PubMed]
  • 17. International Special dietary Foods Industries. Background information on Enterobacter sakazakii. 2004;
  • 18. Mardaneh J, Soltan-Dallal MM. Isolation and Identification of E. cowanii from Powdered Infant Formula in NICU and Determination of Antimicrobial Susceptibility of Isolates. Iran J Pediatr. 2014; 24(3): 261-6[PubMed]
  • 19. Poorabbas B, Mardaneh J, Rezaei Z, Kalani M, Pouladfar G, Alami MH, et al. Nosocomial Infections: Multicenter surveillance of antimicrobial resistance profile of Staphylococcus aureus and Gram negative rods isolated from blood and other sterile body fluids in Iran. Iran J Microbiol. 2015; 7(3): 127-35[PubMed]
  • 20. Keyser M, Witthuhn RC, Ronquest LC, Britz TJ. Treatment of winery effluent with upflow anaerobic sludge blanket (UASB)--granular sludges enriched with Enterobacter sakazakii. Biotechnol Lett. 2003; 25(22): 1893-8[PubMed]
  • 21. Mitov I, Strateva T, Markova B. Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of pseudomonas aeruginosa. Braz J Microbiol. 2010; 41(3): 588-95[DOI][PubMed]
  • 22. Masood N, Moore K, Farbos A, Paszkiewicz K, Dickins B, McNally A, et al. Genomic dissection of the 1994 Cronobacter sakazakii outbreak in a French neonatal intensive care unit. BMC Genomics. 2015; 16: 750[DOI][PubMed]
  • 23. Microbiological testing in food safety management. 2002;
  • 24. Kilonzo-Nthenge A, Rotich E, Godwin S, Nahashon S, Chen F. Prevalence and antimicrobial resistance of Cronobacter sakazakii isolated from domestic kitchens in middle Tennessee, United States. J Food Prot. 2012; 75(8): 1512-7[DOI][PubMed]
  • 25. Kim K, Jang SS, Kim SK, Park JH, Heu S, Ryu S. Prevalence and genetic diversity of Enterobacter sakazakii in ingredients of infant foods. Int J Food Microbiol. 2008; 122(1-2): 196-203[DOI][PubMed]
  • 26. Ahmadi K, Farajzadeh Sheikh A, Mardaneh J, Modarresi F, Shoja S. Detection of Enterobacter sakazakii in neonatal sepsis by PCR on 16S ribosomal RNA. Iranian South Med J. 2014; 17(3): 272-9
  • 27. Reich F, Konig R, von Wiese W, Klein G. Prevalence of Cronobacter spp. in a powdered infant formula processing environment. Int J Food Microbiol. 2010; 140(2-3): 214-7[DOI][PubMed]
  • 28. Masood N, Moore K, Farbos A, Hariri S, Paszkiewicz K, Dickins B, et al. Draft Genome Sequences of Three Newly Identified Species in the Genus Cronobacter, C. helveticus LMG23732T, C. pulveris LMG24059, and C. zurichensis LMG23730T. Genome Announc. 2013; 1(5)[DOI][PubMed]
  • 29. Farber JM. Enterobacter sakazakii--new foods for thought? Lancet. 2004; 363(9402): 5-6[DOI][PubMed]
  • 30. Mardaneh J, Soltan Dallal MM, Taheripoor M, Rajabi Z. Isolation, Identification and Antimicrobial Susceptibility Pattern of Tatumella ptyseos Strains Isolated From Powdered Infant Formula Milk Consumed in Neonatal Intensive Care Unit: First Report From Iran. Jundishapur J Microbiol. 2014; 7(6): 10608[DOI][PubMed]
  • 31. Mardaneh J, Dallal MM. Isolation, identification and antimicrobial susceptibility of Pantoea (Enterobacter) agglomerans isolated from consumed powdered infant formula milk (PIF) in NICU ward: First report from Iran. Iran J Microbiol. 2013; 5(3): 263-7[PubMed]
  • 32. Shaghaghian S, Pourabbas B, Alborzi A, Askarian M, Mardaneh J. Vancomycin-Resistant Entrococci colonization in chronic hemodialysis patients and its risk factors in southern Iran (2005-2006). Iran Red Crescent Med J. 2012; 14(10): 686-91[PubMed]
  • 33. Anvarinejad M, Pouladfar GR, Pourabbas B, Amin Shahidi M, Rafaatpour N, Dehyadegari MA, et al. Detection of Salmonella spp. with the BACTEC 9240 Automated Blood Culture System in 2008 - 2014 in Southern Iran (Shiraz): Biogrouping, MIC, and Antimicrobial Susceptibility Profiles of Isolates. Jundishapur J Microbiol. 2016; 9(4): 26505[DOI][PubMed]
  • 34. Mardaneh J, Soltan Dallal MM. Isolation and Identification Enterobacter asburiae from Consumed Powdered Infant Formula Milk (PIF) in the Neonatal Intensive Care Unit (NICU). Acta Med Iran. 2016; 54(1): 39-43[PubMed]
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