Archives of Pediatric Infectious Diseases

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Strain Typing and Molecular Characterization of CTX-M-1 Group ESBL in Clinical Klebsiella pneumoniae Isolated from Children

Shahin Najar Peerayeh 1 , Safoura Derakhshan 2 , * , Fatemeh Fallah 3 and Bita Bakhshi 1
Authors Information
1 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
2 Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
3 Pediatric Infections Research Center, Mofid Children’s Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Article information
  • Archives of Pediatric Infectious Diseases: April 01, 2017, 5 (2); e39193
  • Published Online: September 5, 2016
  • Article Type: Research Article
  • Received: May 14, 2016
  • Revised: July 25, 2016
  • Accepted: August 20, 2016
  • DOI: 10.5812/pedinfect.39193

To Cite: Najar Peerayeh S, Derakhshan S, Fallah F, Bakhshi B. Strain Typing and Molecular Characterization of CTX-M-1 Group ESBL in Clinical Klebsiella pneumoniae Isolated from Children, Arch Pediatr Infect Dis. 2017 ; 5(2):e39193. doi: 10.5812/pedinfect.39193.

Abstract
Copyright © 2016, Pediartric Infections Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Manzur A, Tubau F, Pujol M, Calatayud L, Dominguez MA, Pena C, et al. Nosocomial outbreak due to extended-spectrum-beta-lactamase- producing Enterobacter cloacae in a cardiothoracic intensive care unit. J Clin Microbiol. 2007; 45(8): 2365-9[DOI][PubMed]
  • 2. Abbassi MS, Torres C, Achour W, Vinue L, Saenz Y, Costa D, et al. Genetic characterisation of CTX-M-15-producing Klebsiella pneumoniae and Escherichia coli strains isolated from stem cell transplant patients in Tunisia. Int J Antimicrob Agents. 2008; 32(4): 308-14[DOI][PubMed]
  • 3. Poirel L, Gniadkowski M, Nordmann P. Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum beta-lactamase CTX-M-15 and of its structurally related beta-lactamase CTX-M-3. J Antimicrob Chemother. 2002; 50(6): 1031-4[PubMed]
  • 4. Lee MY, Ko KS, Kang CI, Chung DR, Peck KR, Song JH. High prevalence of CTX-M-15-producing Klebsiella pneumoniae isolates in Asian countries: diverse clones and clonal dissemination. Int J Antimicrob Agents. 2011; 38(2): 160-3[DOI][PubMed]
  • 5. Iroha IR, Esimone CO, Neumann S, Marlinghaus L, Korte M, Szabados F, et al. First description of Escherichia coli producing CTX-M-15- extended spectrum beta lactamase (ESBL) in out-patients from south eastern Nigeria. Ann Clin Microbiol Antimicrob. 2012; 11: 19[DOI][PubMed]
  • 6. Peerayeh SN, Rostami E, Siadat SD, Derakhshan S. High rate of aminoglycoside resistance in CTX-M-15 producing Klebsiella pneumoniae isolates in Tehran, Iran. Lab Med. 2014; 45(3): 231-7[DOI][PubMed]
  • 7. Hernandez JR, Martinez-Martinez L, Canton R, Coque TM, Pascual A, Spanish Group for Nosocomial I. Nationwide study of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum beta-lactamases in Spain. Antimicrob Agents Chemother. 2005; 49(5): 2122-5[DOI][PubMed]
  • 8. Pena C, Pujol M, Ardanuy C, Ricart A, Pallares R, Linares J, et al. An outbreak of hospital-acquired Klebsiella pneumoniae bacteraemia, including strains producing extended-spectrum beta-lactamase. J Hosp Infect. 2001; 47(1): 53-9[DOI][PubMed]
  • 9. Mshana SE, Hain T, Domann E, Lyamuya EF, Chakraborty T, Imirzalioglu C. Predominance of Klebsiella pneumoniae ST14 carrying CTX-M-15 causing neonatal sepsis in Tanzania. BMC Infect Dis. 2013; 13: 466[DOI][PubMed]
  • 10. Vo AT, van Duijkeren E, Gaastra W, Fluit AC. Antimicrobial resistance, class 1 integrons, and genomic island 1 in Salmonella isolates from Vietnam. PLoS One. 2010; 5(2): 9440[DOI][PubMed]
  • 11. Naseer U, Haldorsen B, Tofteland S, Hegstad K, Scheutz F, Simonsen GS, et al. Molecular characterization of CTX-M-15-producing clinical isolates of Escherichia coli reveals the spread of multidrug-resistant ST131 (O25:H4) and ST964 (O102:H6) strains in Norway. APMIS. 2009; 117(7): 526-36[DOI][PubMed]
  • 12. Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005; 63(3): 219-28[DOI][PubMed]
  • 13. Sekse C, Sunde M, Lindstedt BA, Hopp P, Bruheim T, Cudjoe KS, et al. Potentially human-pathogenic Escherichia coli O26 in Norwegian sheep flocks. Appl Environ Microbiol. 2011; 77(14): 4949-58[DOI][PubMed]
  • 14. Derakhshan S, Peerayeh SN, Fallah F, Bakhshi B, Rahbar M, Ashrafi A. Detection of class 1, 2, and 3 integrons among Klebsiella pneumoniae isolated from children in Tehran hospitals. Arch Pediatr Infect Dis. 2013; 1(4): 164-8
  • 15. Saladin M, Cao VT, Lambert T, Donay JL, Herrmann JL, Ould-Hocine Z, et al. Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals. FEMS Microbiol Lett. 2002; 209(2): 161-8[PubMed]
  • 16. Eckert C, Gautier V, Arlet G. DNA sequence analysis of the genetic environment of various blaCTX-M genes. J Antimicrob Chemother. 2006; 57(1): 14-23[DOI][PubMed]
  • 17. Park YJ, Park SY, Oh EJ, Park JJ, Lee KY, Woo GJ, et al. Occurrence of extended-spectrum beta-lactamases among chromosomal AmpC-producing Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens in Korea and investigation of screening criteria. Diagn Microbiol Infect Dis. 2005; 51(4): 265-9[DOI][PubMed]
  • 18. Ribot EM, Fair MA, Gautom R, Cameron DN, Hunter SB, Swaminathan B, et al. Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog Dis. 2006; 3(1): 59-67[DOI][PubMed]
  • 19. Elberse KE, Nunes S, Sa-Leao R, van der Heide HG, Schouls LM. Multiple-locus variable number tandem repeat analysis for Streptococcus pneumoniae: comparison with PFGE and MLST. PLoS One. 2011; 6(5): 19668[DOI][PubMed]
  • 20. Karim A, Poirel L, Nagarajan S, Nordmann P. Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol Lett. 2001; 201(2): 237-41[PubMed]
  • 21. Nicolas-Chanoine MH, Blanco J, Leflon-Guibout V, Demarty R, Alonso MP, Canica MM, et al. Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15. J Antimicrob Chemother. 2008; 61(2): 273-81[DOI][PubMed]
  • 22. Coque TM, Novais A, Carattoli A, Poirel L, Pitout J, Peixe L, et al. Dissemination of clonally related Escherichia coli strains expressing extended-spectrum beta-lactamase CTX-M-15. Emerg Infect Dis. 2008; 14(2): 195-200[DOI][PubMed]
  • 23. Feizabadi MM, Mahamadi-Yeganeh S, Mirsalehian A, Mirafshar SM, Mahboobi M, Nili F, et al. Genetic characterization of ESBL producing strains of Klebsiella pneumoniae from Tehran hospitals. J Infect Dev Ctries. 2010; 4(10): 609-15[PubMed]
  • 24. Peymani A, Farivar TN, Sanikhani R, Javadi A, Najafipour R. Emergence of TEM, SHV, and CTX-M-extended spectrum beta-lactamases and class 1 integron among Enterobacter cloacae isolates collected from hospitals of Tehran and Qazvin, Iran. Microb Drug Resist. 2014; 20(5): 424-30[DOI][PubMed]
  • 25. Alizade H, Fallah F, Ghanbarpour R, Aflatoonian MR, Goudarzi H, Sharifi H. Phylogenetic groups, extended-spectrum beta-lactamases and metallo-beta-lactamase in Escherichia coli isolated from fecal samples of patients with diarrhea in Iran. Gastroenterol Hepatol Bed Bench. 2015; 8(3): 207-14[PubMed]
  • 26. Bagattini M, Crivaro V, Di Popolo A, Gentile F, Scarcella A, Triassi M, et al. Molecular epidemiology of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal intensive care unit. J Antimicrob Chemother. 2006; 57(5): 979-82[DOI][PubMed]
  • 27. Machado E, Coque TM, Canton R, Baquero F, Sousa JC, Peixe L, et al. Dissemination in Portugal of CTX-M-15-, OXA-1-, and TEM-1-producing Enterobacteriaceae strains containing the aac(6')-Ib-cr gene, which encodes an aminoglycoside- and fluoroquinolone-modifying enzyme. Antimicrob Agents Chemother. 2006; 50(9): 3220-1[DOI][PubMed]
  • 28. Habeeb MA, Haque A, Iversen A, Giske CG. Occurrence of virulence genes, 16S rRNA methylases, and plasmid-mediated quinolone resistance genes in CTX-M-producing Escherichia coli from Pakistan. Eur J Clin Microbiol Infect Dis. 2014; 33(3): 399-409[DOI][PubMed]
  • 29. Jouini A, Vinue L, Slama KB, Saenz Y, Klibi N, Hammami S, et al. Characterization of CTX-M and SHV extended-spectrum beta-lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia. J Antimicrob Chemother. 2007; 60(5): 1137-41[DOI][PubMed]
  • 30. Tayh G, Ben Sallem R, Ben Yahia H, Gharsa H, Klibi N, Boudabous A, et al. First Report of Extended-Spectrum beta-Lactamases Among Clinical Isolates of Klebsiella pneumoniae in Gaza Strip, Palestine. Microb Drug Resist. 2016; [DOI][PubMed]
  • 31. Carattoli A. Resistance plasmid families in Enterobacteriaceae. Antimicrob Agents Chemother. 2009; 53(6): 2227-38[DOI][PubMed]
  • 32. Kamatchi C, Sumathi G, Vaidyanathan R. A pilot study on replicon typing of plasmids associated with ESBL genes in Klebsiella pneumoniae from Chennai. Advances BioTech. 2011; 11: 5-10
  • 33. Agyekum A, Fajardo-Lubian A, Ansong D, Partridge SR, Agbenyega T, Iredell JR. blaCTX-M-15 carried by IncF-type plasmids is the dominant ESBL gene in Escherichia coli and Klebsiella pneumoniae at a hospital in Ghana. Diagn Microbiol Infect Dis. 2016; 84(4): 328-33[DOI][PubMed]
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