Archives of Pediatric Infectious Diseases

Published by: Kowsar

Evaluation of Sensitivity of Molecular Methods for Detection of Rifampin-Resistant Strains Amongst Drug-resistant Mycobacterium tuberculosis Isolates

Mahdi Behdani 1 , Hoshang Rafatpanah 2 , Alireza Mohammadzadeh 3 , * , Kiarash Ghazvini 4 , Mostafa Amiri 5 and Jalal Mardaneh 3
Authors Information
1 Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab., Pasteur Institute of Iran, Tehran, IR Iran
2 Immunology Department, Mashhad University of Medical Sciences, Mashhad, IR Iran
3 Microbiology Department, School of Medicine, Gonabad University of Medical Sciences, Gonabad, IR Iran
4 Mycobacteriology Department, Microbiology and Virology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran
5 Basic Sciences Department, Faculty of Para Medicine Sciences, Gonabad University of Medical Sciences, Gonabad, IR Iran
Article information
  • Archives of Pediatric Infectious Diseases: July 2017, 5 (3); e40580
  • Published Online: October 16, 2016
  • Article Type: Research Article
  • Received: July 3, 2016
  • Revised: September 12, 2016
  • Accepted: October 1, 2016
  • DOI: 10.5812/pedinfect.40580

To Cite: Behdani M, Rafatpanah H, Mohammadzadeh A, Ghazvini K, Amiri M, et al. Evaluation of Sensitivity of Molecular Methods for Detection of Rifampin-Resistant Strains Amongst Drug-resistant Mycobacterium tuberculosis Isolates, Arch Pediatr Infect Dis. 2017 ; 5(3):e40580. doi: 10.5812/pedinfect.40580.

Copyright © 2016, Pediartric Infections Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Zumla A, Raviglione M, Hafner R, von Reyn CF. Tuberculosis. N Engl J Med. 2013; 368(8): 745-55[DOI][PubMed]
  • 2. Regional office for the eastern mediterranean: Islamic Republic of Iran profile:World Health Organization. 2015;
  • 3. Palomino JC, Martin A. Drug Resistance Mechanisms in Mycobacterium tuberculosis. Antibiotics (Basel). 2014; 3(3): 317-40[DOI][PubMed]
  • 4. Jassal M, Bishai WR. Extensively drug-resistant tuberculosis. Lancet Infect Dis. 2009; 9(1): 19-30[DOI][PubMed]
  • 5. Madariaga MG, Lalloo UG, Swindells S. Extensively drug-resistant tuberculosis. Am J Med. 2008; 121(10): 835-44[DOI][PubMed]
  • 6. Ahmed I, Jabeen K, Inayat R, Hasan R. Susceptibility testing of extensively drug-resistant and pre-extensively drug-resistant Mycobacterium tuberculosis against levofloxacin, linezolid, and amoxicillin-clavulanate. Antimicrob Agents Chemother. 2013; 57(6): 2522-5[DOI][PubMed]
  • 7. Global tuberculosis report. 2015;
  • 8. Global tuberculosis control: WHO report. 2011;
  • 9. Masjedi MR, Farnia P, Sorooch S, Pooramiri MV, Mansoori SD, Zarifi AZ, et al. Extensively drug-resistant tuberculosis: 2 years of surveillance in Iran. Clin Infect Dis. 2006; 43(7): 841-7[DOI][PubMed]
  • 10. Jacobson KR, Tierney DB, Jeon CY, Mitnick CD, Murray MB. Treatment outcomes among patients with extensively drug-resistant tuberculosis: systematic review and meta-analysis. Clin Infect Dis. 2010; 51(1): 6-14[DOI][PubMed]
  • 11. Blanchard JS. Molecular mechanisms of drug resistance in Mycobacterium tuberculosis. Annu Rev Biochem. 1996; 65: 215-39[DOI][PubMed]
  • 12. Mokrousov I, Otten T, Vyshnevskiy B, Narvskaya O. Allele-specific rpoB PCR assays for detection of rifampin-resistant Mycobacterium tuberculosis in sputum smears. Antimicrob Agents Chemother. 2003; 47(7): 2231-5[PubMed]
  • 13. Chia BS, Lanzas F, Rifat D, Herrera A, Kim EY, Sailer C, et al. Use of multiplex allele-specific polymerase chain reaction (MAS-PCR) to detect multidrug-resistant tuberculosis in Panama. PLoS One. 2012; 7(7): 40456[DOI][PubMed]
  • 14. Palomino JC, Martin A, Portaels F. Rapid drug resistance detection in Mycobacterium tuberculosis: a review of colourimetric methods. Clin Microbiol Infect. 2007; 13(8): 754-62[DOI][PubMed]
  • 15. Mohammadzadeh A, Farnia P, Ghazvini K, Behdani M, Rashed T, Ghanaat J. Rapid and low-cost colorimetric method using 2,3,5-triphenyltetrazolium chloride for detection of multidrug-resistant Mycobacterium tuberculosis. J Med Microbiol. 2006; 55: 1657-9[DOI][PubMed]
  • 16. Fan XY, Hu ZY, Xu FH, Yan ZQ, Guo SQ, Li ZM. Rapid detection of rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis isolates in shanghai by using the amplification refractory mutation system. J Clin Microbiol. 2003; 41(3): 993-7[PubMed]
  • 17. Gupta A, Prakash P, Singh SK, Anupurba S. Rapid genotypic detection of rpoB and katG gene mutations in Mycobacterium tuberculosis clinical isolates from Northern India as determined by MAS-PCR. J Clin Lab Anal. 2013; 27(1): 31-7[DOI][PubMed]
  • 18. Kim BJ, Kim SY, Park BH, Lyu MA, Park IK, Bai GH, et al. Mutations in the rpoB gene of Mycobacterium tuberculosis that interfere with PCR-single-strand conformation polymorphism analysis for rifampin susceptibility testing. J Clin Microbiol. 1997; 35(2): 492-4[PubMed]
  • 19. Grutzmacher LK, Dalmarco EM, Blatt SL, Cordova CM. Drug resistance of Mycobacterium tuberculosis strains in southern Brazil. Rev Soc Bras Med Trop. 2012; 45(1): 95-9[PubMed]
  • 20. Miyata M, Pavan FR, Sato DN, Marino LB, Hirata MH, Cardoso RF, et al. Drug resistance in Mycobacterium tuberculosis clinical isolates from Brazil: phenotypic and genotypic methods. Biomed Pharmacother. 2011; 65(6): 456-9[DOI][PubMed]
  • 21. Kocagoz T, Saribas Z, Alp A. Rapid determination of rifampin resistance in clinical isolates of Mycobacterium tuberculosis by real-time PCR. J Clin Microbiol. 2005; 43(12): 6015-9[DOI][PubMed]
  • 22. Soudani A, Hadjfredj S, Zribi M, Masmoudi A, Messaoud T, Tiouri H, et al. Characterization of Tunisian Mycobacterium tuberculosis rifampin-resistant clinical isolates. J Clin Microbiol. 2007; 45(9): 3095-7[DOI][PubMed]
  • 23. Zeka AN, Tasbakan S, Cavusoglu C. Evaluation of the GeneXpert MTB/RIF assay for rapid diagnosis of tuberculosis and detection of rifampin resistance in pulmonary and extrapulmonary specimens. J Clin Microbiol. 2011; 49(12): 4138-41[DOI][PubMed]
  • 24. Sheikholslami MF, Farnia P, Tabarsi P, Aghali Merza M, Valiollah Pour Amiri M, Mohammadi F. Comparison of polymerase chain reaction single-strand conformation polymorphism with DNA sequencing to detect drug resistance of Mycobacterium tuberculosis isolates. Iran J Clin Infect Dis. 2011; 6(2): 66-70
  • 25. Boehme CC, Nabeta P, Hillemann D, Nicol MP, Shenai S, Krapp F, et al. Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med. 2010; 363(11): 1005-15[DOI][PubMed]
  • 26. Sahebi L, Khalil Ansarin K, Monfaredan A, Farajnia S, Nili S, Khalili M. Rapid Detection of Rifampicin- and Isoniazid-Resistant Mycobacterium tuberculosis Using Real-Time PCR. Jundishapur J Microbiol. : 29147
  • 27. Anti-tuberculosis drug resistance in the world: third global report. 2004;
  • 28. van Soolingen D, Hermans PW, de Haas PE, Soll DR, van Embden JD. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol. 1991; 29(11): 2578-86[PubMed]
  • 29. Temesgen Z, Satoh K, Uhl JR, Kline BC, Cockerill F3. Use of polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis to detect a point mutation in the catalase-peroxidase gene (katG) of Mycobacterium tuberculosis. Mol Cell Probes. 1997; 11(1): 59-63[DOI][PubMed]
  • 30. Khoshneviszadeh M, Edraki N, Javidnia K, Alborzi A, Pourabbas B, Mardaneh J, et al. Synthesis and biological evaluation of some new 1,4-dihydropyridines containing different ester substitute and diethyl carbamoyl group as anti-tubercular agents. Bioorg Med Chem. 2009; 17(4): 1579-86[DOI][PubMed]
  • 31. Shah NS, Wright A, Bai GH, Barrera L, Boulahbal F, Martin-Casabona N, et al. Worldwide emergence of extensively drug-resistant tuberculosis. Emerg Infect Dis. 2007; 13(3): 380-7[DOI][PubMed]
  • 32. Kam KM, Yip CW. Surveillance of Mycobacterium tuberculosis susceptibility to second-line drugs in Hong Kong, 1995-2002, after the implementation of DOTS-plus. Int J Tuberc Lung Dis. 2004; 8(6): 760-6[PubMed]
  • 33. Sun Z, Chao Y, Zhang X, Zhang J, Li Y, Qiu Y, et al. Characterization of extensively drug-resistant Mycobacterium tuberculosis clinical isolates in China. J Clin Microbiol. 2008; 46(12): 4075-7[DOI][PubMed]
  • 34. WHO Global Task Force outlines measures to combat XDR TB worldwide. 2006;
  • 35. Bahrmand AR, Titov LP, Tasbiti AH, Yari S, Graviss EA. High-level rifampin resistance correlates with multiple mutations in the rpoB gene of pulmonary tuberculosis isolates from the Afghanistan border of Iran. J Clin Microbiol. 2009; 47(9): 2744-50[DOI][PubMed]
  • 36. Cheng X, Zhang J, Yang L, Xu X, Liu J, Yu W, et al. A new Multi-PCR-SSCP assay for simultaneous detection of isoniazid and rifampin resistance in Mycobacterium tuberculosis. J Microbiol Methods. 2007; 70(2): 301-5[DOI][PubMed]
  • 37. Isfahani BN, Tavakoli A, Salehi M, Tazhibi M. Detection of rifampin resistance patterns in Mycobacterium tuberculosis strains isolated in Iran by polymerase chain reaction-single-strand conformation polymorphism and direct sequencing methods. Mem Inst Oswaldo Cruz. 2006; 101(6): 597-602[PubMed]
  • 38. Imani F, Babak F, Fazlollah MS, Nematollah JJ. Rapid detection of MDR-Mycobacterium tuberculosis using modified PCR-SSCP from clinical Specimens. Asian Pac J Trop Biomed. 2014; 4: 165-70[DOI][PubMed]
  • 39. Steingart KR, Schiller I, Horne DJ, Pai M, Boehme CC, Dendukuri N. Xpert(R) MTB/RIF assay for pulmonary tuberculosis and rifampicin resistance in adults. Cochrane Database Syst Rev. 2014; (1): 9593[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments