Archives of Pediatric Infectious Diseases

Published by: Kowsar

Prevalence and Antibiotic Susceptibility Patterns of Salmonella and Shigella Species Isolated from Pediatric Diarrhea in Tehran

Narges Nodeh Farahani 1 , Faramarz Masjedian Jazi 1 , Bahram Nikmanesh 2 , 3 , Parisa Asadolahi 1 , Behrooz Sadeghi Kalani 1 and Nour Amirmozafari 1 , *
Authors Information
1 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2 Zoonosis Research Center, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
Article information
  • Archives of Pediatric Infectious Diseases: October 2018, 6 (4); e57328
  • Published Online: August 11, 2018
  • Article Type: Research Article
  • Received: July 2, 2017
  • Revised: October 8, 2017
  • Accepted: October 31, 2017
  • DOI: 10.5812/pedinfect.57328

To Cite: Nodeh Farahani N, Masjedian Jazi F , Nikmanesh B, Asadolahi P, Sadeghi Kalani B , et al. Prevalence and Antibiotic Susceptibility Patterns of Salmonella and Shigella Species Isolated from Pediatric Diarrhea in Tehran, Arch Pediatr Infect Dis. 2018 ; 6(4):e57328. doi: 10.5812/pedinfect.57328.

Copyright © 2018, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Abbasi P, Kargar M, Doosti A, Mardaneh J, Ghorbani-Dalini S, Dehyadegari MA. Characterization of Shiga-toxin producing E.coli (STEC) and enteropathogenic E.coli (EPEC) using multiplex Real-Time PCR assays for stx1 , stx2 , eaeA. Iran J Microbiol. 2014;6(3):169-74. [PubMed: 25870750]. [PubMed Central: PMC4393493].
  • 2. Ahs JW, Tao W, Löfgren J, Forsberg BC. Diarrheal diseases in low- and middle-income countries: Incidence, prevention and management. Open Infect Dis J. 2010;4(1):113-24. doi: 10.2174/1874279301004010113.
  • 3. Anvarinejad M, Pouladfar GR, Pourabbas B, Amin Shahidi M, Rafaatpour N, Dehyadegari MA, et al. Detection of Salmonella spp. with the BACTEC 9240 Automated Blood Culture System in 2008 - 2014 in Southern Iran (Shiraz): Biogrouping, MIC, and Antimicrobial Susceptibility Profiles of Isolates. Jundishapur J Microbiol. 2016;9(4). e26505. doi: 10.5812/jjm.26505. [PubMed: 27284396]. [PubMed Central: PMC4897598].
  • 4. Pazhani GP, Niyogi SK, Singh AK, Sen B, Taneja N, Kundu M, et al. Molecular characterization of multidrug-resistant Shigella species isolated from epidemic and endemic cases of shigellosis in India. J Med Microbiol. 2008;57(Pt 7):856-63. doi: 10.1099/jmm.0.2008/000521-0. [PubMed: 18566144].
  • 5. Hosseini Nave H, Mansouri S, Sadeghi A, Moradi M. Molecular diagnosis and anti-microbial resistance patterns among Shigella spp. isolated from patients with diarrhea. Gastroenterol Hepatol Bed Bench. 2016;9(3):205-10. [PubMed: 27458513]. [PubMed Central: PMC4947135].
  • 6. Mardaneh J, Abbas Poor S, Afrugh P. Prevalence of Shigella species and antimicrobial resistance patterns of isolated strains from infected pediatrics in Tehran. Int J Enteric Pathog. 2013;1(1):28-31. doi: 10.17795/ijep10705.
  • 7. Ranjbar R, Ghazi FM, Farshad S, Giammanco GM, Aleo A, Owlia P, et al. The occurrence of extended-spectrum beta-lactamase producing Shigella spp. in Tehran, Iran. Iran J Microbiol. 2013;5(2):108-12. [PubMed: 23825726]. [PubMed Central: PMC3696844].
  • 8. Mamuye Y, Metaferia G, Birhanu A, Desta K, Fantaw S. Isolation and antibiotic susceptibility patterns of shigella and salmonella among under 5 children with acute diarrhoea: A cross-sectional study at selected public health facilities in Addis Ababa, Ethiopia. Clin Microbiol. 2015;4(1). doi: 10.4172/2327-5073.1000186.
  • 9. Bialvaei AZ, Pourlak T, Aghamali M, Asgharzadeh M, Gholizadeh P, Kafil HS. The Prevalence of CTX-M-15 Extended-spectrum beta-Lactamases Among Salmonella spp. and Shigella spp. Isolated from three Iranian Hospitals. Eur J Microbiol Immunol (Bp). 2017;7(2):133-7. doi: 10.1556/1886.2017.00004. [PubMed: 28690880]. [PubMed Central: PMC5495085].
  • 10. Abbasi P, Kargar M, Doosti A, Mardaneh J, Ghorbani-Dalini S, Dehyadegari MA. Molecular detection of diffusely adherent escherichia coli strains associated with diarrhea in Shiraz, Iran. Arch Pediatr Infect Dis. 2016;5(2). doi: 10.5812/pedinfect.37629.
  • 11. Tille P. Bailey & Scott's Diagnostic Microbiology-E-Book. Elsevier Health Sciences; 2013.
  • 12. Clinical and Laboratory Standards Institute . Performance standards for antimicrobial susceptibility testing. 26th Informational Supplement. CLSI document M100-S26. Wayne PA: CLSI; 2016.
  • 13. Awole M, Gebre-Selassie S, Kassa T, Kibru G. Isolation of potential bacterial pathogens from the stool of HIV-infected and HIV-non-infected patients and their antmicrobial susceptibility patterns in Jimma Hospital, south west Ethiopia. Ethiop Med J. 2002;40(4):353-64. [PubMed: 12596655].
  • 14. Reda AA, Seyoum B, Yimam J, Fiseha S, Jean-Michel V. Antibiotic susceptibility patterns of Salmonella and Shigella isolates in Harar, Eastern Ethiopia. J Infect Dis Immun. 2011;3(8):134-9.
  • 15. Mache A. Salmonella serogroups and their antibiotic resistance patterns isolated from diarrhoeal stools of pediatric out-patients in Jimma Hospital and Jimma health center, South West Ethiopia. Ethiop J Health Sci. 2002;12(1):37-46.
  • 16. Tiruneh M. Serodiversity and antimicrobial resistance pattern of Shigella isolates at Gondar University teaching hospital, Northwest Ethiopia. Jpn J Infect Dis. 2009;62(2):93-7. [PubMed: 19305047].
  • 17. Samie A, Guerrant RL, Barrett L, Bessong PO, Igumbor EO, Obi CL. Prevalence of intestinal parasitic and bacterial pathogens in diarrhoeal and non-diarroeal human stools from Vhembe district, South Africa. J Health Popul Nutr. 2009;27(6):739-45. [PubMed: 20099757]. [PubMed Central: PMC2928113].
  • 18. Jomezadeh N, Babamoradi S, Kalantar E, Javaherizadeh H. Isolation and antibiotic susceptibility of Shigella species from stool samples among hospitalized children in Abadan, Iran. Gastroenterol Hepatol Bed Bench. 2014;7(4):218-23. [PubMed: 25289136]. [PubMed Central: PMC4185876].
  • 19. Talebreza A, Memariani M, Memariani H, Shirazi MH, Eghbali Shamsabad P, Bakhtiari M. Prevalence and antibiotic susceptibility of shigella species isolated from pediatric patients in Tehran. Arch Pediatr Infect Dis. 2015;4(1). doi: 10.5812/pedinfect.32395.
  • 20. Njunda AL, Assob JC, Nsagha DS, Kamga HL, Awafong MP, Weledji EP. Epidemiological, clinical features and susceptibility pattern of shigellosis in the buea health district, Cameroon. BMC Res Notes. 2012;5:54. doi: 10.1186/1756-0500-5-54. [PubMed: 22264300]. [PubMed Central: PMC3285523].
  • 21. Nath R, Saikia L, Choudhury G, Sharma D. Drug resistant Shigella flexneri in & around Dibrugarh, north-east India. Indian J Med Res. 2013;137(1):183-6. [PubMed: 23481070]. [PubMed Central: PMC3657885].
  • 22. Opintan J, Newman MJ. Distribution of serogroups and serotypes of multiple drug resistant Shigella isolates. Ghana Med J. 2007;41(1):8-29. [PubMed: 17622331]. [PubMed Central: PMC1890532].
  • 23. Hamze M, Osman M, Mallat H, Achkar M. Antibiotic susceptibility of Salmonella spp., Shigella spp. and enteropathogenic Escherichia coli strains isolated from diarrheic children in Tripoli, North Lebanon. Int Arabic J Antimicrob Agents. 2016. doi: 10.3823/787.
  • 24. MoezArdalan K, Zali MR, Dallal MM, Hemami MR, Salmanzadeh-Ahrabi S. Prevalence and pattern of antimicrobial resistance of Shigella species among patients with acute diarrhoea in Karaj, Tehran, Iran. J Health Popul Nutr. 2003;21(2):96-102. [PubMed: 13677436].
  • 25. Tajbakhsh M, Garcia Migura L, Rahbar M, Svendsen CA, Mohammadzadeh M, Zali MR, et al. Antimicrobial-resistant Shigella infections from Iran: an overlooked problem? J Antimicrob Chemother. 2012;67(5):1128-33. doi: 10.1093/jac/dks023. [PubMed: 22345385].
  • 26. Farshad S, Sheikhi R, Japoni A, Basiri E, Alborzi A. Characterization of Shigella strains in Iran by plasmid profile analysis and PCR amplification of ipa genes. J Clin Microbiol. 2006;44(8):2879-83. doi: 10.1128/JCM.00310-06. [PubMed: 16891506]. [PubMed Central: PMC1594633].
  • 27. Eftekhari N, Bakhshi B, Pourshafie MR, Zarbakhsh B, Rahbar M, Hajia M, et al. Genetic diversity of Shigella spp. and their integron content. Foodborne Pathog Dis. 2013;10(3):237-42. doi: 10.1089/fpd.2012.1250. [PubMed: 23489046].
  • 28. Ansari S, Sherchand JB, Parajuli K, Mishra SK, Dahal RK, Shrestha S, et al. Bacterial etiology of acute diarrhea in children under five years of age. J Nepal Health Res Counc. 2012;10(22):218-23. [PubMed: 23281455].
  • 29. Pazhani GP, Ramamurthy T, Mitra U, Bhattacharya SK, Niyogi SK. Species diversity and antimicrobial resistance of Shigella spp. isolated between 2001 and 2004 from hospitalized children with diarrhoea in Kolkata (Calcutta), India. Epidemiol Infect. 2005;133(6):1089-95. doi: 10.1017/S0950268805004498. [PubMed: 16274506]. [PubMed Central: PMC2870343].
  • 30. Pourakbari B, Mamishi S, Mashoori N, Mahboobi N, Ashtiani MH, Afsharpaiman S, et al. Frequency and antimicrobial susceptibility of Shigella species isolated in Children Medical Center Hospital, Tehran, Iran, 2001-2006. Braz J Infect Dis. 2010;14(2):153-7. doi: 10.1016/S1413-8670(10)70029-5. [PubMed: 20563441].
  • 31. Ghosh AR, Sugunan AP, Sehgal SC, Bharadwaj AP. Emergence of nalidixic acid-resistant Shigella sonnei in acute-diarrhea patients on Andaman and Nicobar Islands, India. Antimicrob Agents Chemother. 2003;47(4):1483. doi: 10.1128/AAC.47.4.1483.2003. [PubMed: 12654701]. [PubMed Central: PMC152529].
  • 32. Jesudason MV. Shigella isolation in Vellore, south India (1997-2001). Indian J Med Res. 2002;115:11-3. [PubMed: 12424931].
  • 33. Barari Savadkoohi R, Ahmadpour-Kacho M. Prevalence of Shigella species and their antimicrobial resistance patterns at Amirkola children hospital, North of Iran. Iran J Pediatr. 2007;17(2):118-22.
  • 34. Sakhaei A, Savari M, Shokoohizadeh L, Hadian M, Ekrami A. Characterization of shigella strains by plasmid profile analysis and antibiotic susceptibility patterns in a pediatric Hospital in Ahvaz. Int J Enteric Pathog. 2015;3(4). doi: 10.17795/ijep29924.
  • 35. Mardaneh J, Soltan Dallal MM. Study of cronobacter sakazakii strains isolated from powdered milk infant formula by phenotypic and molecular methods in Iran. Arch Pediatr Infect Dis. 2017;5(1).

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