Archives of Pediatric Infectious Diseases

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Evaluation of the Virulence Features and Antibiotic Resistance Patterns of Pathogenic Pseudomonas aeruginosa Strains Isolated from Hospitalized Patients in Gonabad, Iran

Alireza Mohammadzadeh 1 , Jalal Mardaneh 1 , Reza Ahmadi 2 , * and Javad Adabi 3
Authors Information
1 Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, IR Iran
2 Department of Internal Medicine, School of Medicine, Gonabad University of Medical Sciences, Gonabad, IR Iran
3 Microbiology Laboratory, 22Bahman Hospital, Gonabad University of Medical Sciences, Gonabad, IR Iran
Article information
  • Archives of Pediatric Infectious Diseases: July 2017, 5 (3); e41267
  • Published Online: January 16, 2017
  • Article Type: Research Article
  • Received: August 7, 2016
  • Revised: October 2, 2016
  • Accepted: October 29, 2016
  • DOI: 10.5812/pedinfect.41267

To Cite: Mohammadzadeh A, Mardaneh J, Ahmadi R, Adabi J. Evaluation of the Virulence Features and Antibiotic Resistance Patterns of Pathogenic Pseudomonas aeruginosa Strains Isolated from Hospitalized Patients in Gonabad, Iran, Arch Pediatr Infect Dis. 2017 ; 5(3):e41267. doi: 10.5812/pedinfect.41267.

Copyright © 2017, Pediartric Infections Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev. 2009; 22(4): 582-610[DOI][PubMed]
  • 2. Schwartz T, Armant O, Bretschneider N, Hahn A, Kirchen S, Seifert M, et al. Whole genome and transcriptome analyses of environmental antibiotic sensitive and multi-resistant Pseudomonas aeruginosa isolates exposed to waste water and tap water. Microb Biotechnol. 2015; 8(1): 116-30[DOI][PubMed]
  • 3. Anvarinejad M, Japoni A, Rafaatpour N, Mardaneh J, Abbasi P, Amin Shahidi M, et al. Burn Patients Infected With Metallo-Beta-Lactamase-Producing Pseudomonas aeruginosa: Multidrug-Resistant Strains. Arch Trauma Res. 2014; 3(2)[DOI][PubMed]
  • 4. Poorabbas B, Mardaneh J, Rezaei Z, Kalani M, Pouladfar G, Alami MH, et al. Nosocomial Infections: Multicenter surveillance of antimicrobial resistance profile of Staphylococcus aureus and Gram negative rods isolated from blood and other sterile body fluids in Iran. Iran J Microbiol. 2015; 7(3): 127-35[PubMed]
  • 5. Soltani J, Poorabbas B, Miri N, Mardaneh J. Health care associated infections, antibiotic resistance and clinical outcome: A surveillance study from Sanandaj, Iran. World J Clin Cases. 2016; 4(3): 63-70[DOI][PubMed]
  • 6. Lanotte P, Watt S, Mereghetti L, Dartiguelongue N, Rastegar-Lari A, Goudeau A, et al. Genetic features of Pseudomonas aeruginosa isolates from cystic fibrosis patients compared with those of isolates from other origins. J Med Microbiol. 2004; 53: 73-81[DOI][PubMed]
  • 7. Chen J, Su Z, Liu Y, Wang S, Dai X, Li Y, et al. Identification and characterization of class 1 integrons among Pseudomonas aeruginosa isolates from patients in Zhenjiang, China. Int J Infect Dis. 2009; 13(6): 717-21[DOI][PubMed]
  • 8. Perry J, Waglechner N, Wright G. The Prehistory of Antibiotic Resistance. Cold Spring Harb Perspect Med. 2016; 6(6)[DOI][PubMed]
  • 9. Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st century. Perspect Medicin Chem. 2014; 6: 25-64[DOI][PubMed]
  • 10. Dumas JL, van Delden C, Perron K, Kohler T. Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR. FEMS Microbiol Lett. 2006; 254(2): 217-25[DOI][PubMed]
  • 11. Mesaros N, Nordmann P, Plesiat P, Roussel-Delvallez M, Van Eldere J, Glupczynski Y, et al. Pseudomonas aeruginosa: resistance and therapeutic options at the turn of the new millennium. Clin Microbiol Infect. 2007; 13(6): 560-78[DOI][PubMed]
  • 12. Quale J, Bratu S, Gupta J, Landman D. Interplay of efflux system, ampC, and oprD expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates. Antimicrob Agents Chemother. 2006; 50(5): 1633-41[DOI][PubMed]
  • 13. Hirsch EB, Tam VH. Impact of multidrug-resistant Pseudomonas aeruginosa infection on patient outcomes. Expert Rev Pharmacoecon Outcomes Res. 2010; 10(4): 441-51[DOI][PubMed]
  • 14. M100-S24. 2014;
  • 15. Khan AA, Cerniglia CE. Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR. Appl Environ Microbiol. 1994; 60(10): 3739-45[PubMed]
  • 16. Yang JL, Wang MS, Cheng AC, Pan KC, Li CF, Deng SX. A simple and rapid method for extracting bacterial DNA from intestinal microflora for ERIC-PCR detection. World J Gastroenterol. 2008; 14(18): 2872-6[PubMed]
  • 17. Lin D, Foley SL, Qi Y, Han J, Ji C, Li R, et al. Characterization of antimicrobial resistance of Pseudomonas aeruginosa isolated from canine infections. J Appl Microbiol. 2012; 113(1): 16-23[DOI][PubMed]
  • 18. Mitov I, Strateva T, Markova B. Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of pseudomonas aeruginosa. Braz J Microbiol. 2010; 41(3): 588-95[DOI][PubMed]
  • 19. Nathwani D, Raman G, Sulham K, Gavaghan M, Menon V. Clinical and economic consequences of hospital-acquired resistant and multidrug-resistant Pseudomonas aeruginosa infections: a systematic review and meta-analysis. Antimicrob Resist Infect Control. 2014; 3(1): 32[DOI][PubMed]
  • 20. Franco MR, Caiaffa-Filho HH, Burattini MN, Rossi F. Metallo-beta-lactamases among imipenem-resistant Pseudomonas aeruginosa in a Brazilian university hospital. Clinics (Sao Paulo). 2010; 65(9): 825-9[PubMed]
  • 21. Raja NS, Singh NN. Antimicrobial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital. J Microbiol Immunol Infect. 2007; 40(1): 45-9[PubMed]
  • 22. Fatima A, Naqvi SB, Khaliq SA, Perveen S, Jabeen S. Antimicrobial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa isolated from patients of lower respiratory tract infections. Springerplus. 2012; 1(1): 70[DOI][PubMed]
  • 23. Jafari M, Fallah F, Borhan RS, Navidinia M, Karimi A, Tabatabaei S, et al. The first report of CMY, aac (6′)-Ib and 16S rRNA methylase genes among Pseudomonas aeruginosa isolates from Iran. Arch Pediatr Infect Dis. 2013; 1(3): 109-12
  • 24. Gu B, Tong M, Zhao W, Liu G, Ning M, Pan S, et al. Prevalence and characterization of class I integrons among Pseudomonas aeruginosa and Acinetobacter baumannii isolates from patients in Nanjing, China. J Clin Microbiol. 2007; 45(1): 241-3[DOI][PubMed]
  • 25. Navidinia M. The clinical importance of emerging ESKAPE pathogens in nosocomial infections. J Param Sci. 2016; 7(3)
  • 26. Nateghian A, Robinson J, Vosough P, Navidinia M, Malekan M, Mehrvar A, et al. Comparison of Antimicrobial Sensitivity to Older and Newer Quinolones versus Piperacillin-Tazobactam, Cefepime and Meropenem in Febrile Patients with Cancer in two Referral Pediatric Centers in Tehran, Iran. Mediterr J Hematol Infect Dis. 2014; 6(1)[DOI][PubMed]
  • 27. Meletis G, Exindari M, Vavatsi N, Sofianou D, Diza E. Mechanisms responsible for the emergence of carbapenem resistance in Pseudomonas aeruginosa. Hippokratia. 2012; 16(4): 303-7[PubMed]
  • 28. Vaez H, Faghri J, Nasr Esfahani B, Moghim S, Fazeli H, Sedighi M, et al. Antibiotic Resistance Patterns and Genetic Diversity in Clinical Isolates of Pseudomonas aeruginosa Isolated From Patients of a Referral Hospital, Isfahan, Iran. Jundishapur J Microbiol. 2015; 8(8)[DOI][PubMed]
  • 29. Mardaneh J, Soltan Dallal MM, Taheripoor M, Rajabi Z. Isolation, Identification and Antimicrobial Susceptibility Pattern of Tatumella ptyseos Strains Isolated From Powdered Infant Formula Milk Consumed in Neonatal Intensive Care Unit: First Report From Iran. Jundishapur J Microbiol. 2014; 7(6)[DOI][PubMed]
  • 30. Shaghaghian S, Pourabbas B, Alborzi A, Askarian M, Mardaneh J. Vancomycin-Resistant Entrococci colonization in chronic hemodialysis patients and its risk factors in southern Iran (2005-2006). Iran Red Crescent Med J. 2012; 14(10): 686-91[PubMed]
  • 31. Mardaneh J, Soltan-Dallal MM. Isolation and Identification of E. cowanii from Powdered Infant Formula in NICU and Determination of Antimicrobial Susceptibility of Isolates. Iran J Pediatr. 2014; 24(3): 261-6[PubMed]
  • 32. Mardaneh J, Dallal MM. Isolation, identification and antimicrobial susceptibility of Pantoea (Enterobacter) agglomerans isolated from consumed powdered infant formula milk (PIF) in NICU ward: First report from Iran. Iran J Microbiol. 2013; 5(3): 263-7[PubMed]
  • 33. Anvarinejad M, Pouladfar GR, Pourabbas B, Amin Shahidi M, Rafaatpour N, Dehyadegari MA, et al. Detection of Salmonella spp. with the BACTEC 9240 Automated Blood Culture System in 2008 - 2014 in Southern Iran (Shiraz): Biogrouping, MIC, and Antimicrobial Susceptibility Profiles of Isolates. Jundishapur J Microbiol. 2016; 9(4)[DOI][PubMed]
  • 34. Mardaneh J, Soltan Dallal MM. Isolation and Identification Enterobacter asburiae from Consumed Powdered Infant Formula Milk (PIF) in the Neonatal Intensive Care Unit (NICU). Acta Med Iran. 2016; 54(1): 39-43[PubMed]
  • 35. Mardaneh J, Soltan Dallal MM. Study of Cronobacter sakazakii Strains Isolated from Powdered Milk Infant Formula by Phenotypic and Molecular Methods in Iran. Arch Pediatr Infect Dis. 2016; 5(1)[DOI]
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