Archives of Pediatric Infectious Diseases

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Detection and Genotyping of Viral Gastroenteritis in Hospitalized Children Below Five Years Old in Cairo, Egypt

Abdou Kamal Allayeh 1 , 2 , * , Reham Mostafa El Baz 3 , Norehan Mohamed Saeed 4 and Mohamed El Sayed Osman 3
Authors Information
1 Virology Lab, Water Pollution Department, National Research Centre, 12622 Dokki, Giza, Egypt
2 Urology Research Department, University Hospital of Zurich, Wagistrasse, Schlieren, Switzerland
3 Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan, Cairo, Egypt
4 Regional Center for Blood Transfusion, Al Malek Al Saleh, Cairo, Egypt
Article information
  • Archives of Pediatric Infectious Diseases: July 2018, 6 (3); e60288
  • Published Online: June 20, 2018
  • Article Type: Research Article
  • Received: August 15, 2017
  • Revised: November 20, 2017
  • Accepted: December 2, 2017
  • DOI: 10.5812/pedinfect.60288

To Cite: Kamal Allayeh A, Mostafa El Baz R, Mohamed Saeed N, El Sayed Osman M. Detection and Genotyping of Viral Gastroenteritis in Hospitalized Children Below Five Years Old in Cairo, Egypt, Arch Pediatr Infect Dis. 2018 ; 6(3):e60288. doi: 10.5812/pedinfect.60288.

Copyright © 2018, Archives of Pediatric Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Neuzil KM, Armah GE, Parashar UD, Duncan Steele A. Rotavirus in Africa: shifting the focus to disease prevention. The University of Chicago Press; 2010.
  • 2. Logan C, O'Leary JJ, O'Sullivan N. Real-time reverse transcription-PCR for detection of rotavirus and adenovirus as causative agents of acute viral gastroenteritis in children. J Clin Microbiol. 2006;44(9):3189-95. doi: 10.1128/JCM.00915-06. [PubMed: 16954246]. [PubMed Central: PMC1594742].
  • 3. Phan TG, Trinh QD, Yagyu F, Okitsu S, Ushijima H. Emergence of rare sapovirus genotype among infants and children with acute gastroenteritis in Japan. Eur J Clin Microbiol Infect Dis. 2007;26(1):21-7. doi: 10.1007/s10096-006-0235-7. [PubMed: 17200841].
  • 4. Jaff DO, Aziz TAG, Smith NR. The Incidence of Rotavirus and Adenovirus Infections among Children with Diarrhea in Sulaimani Province, Iraq. J Biosci Med. 2015;4(1):124.
  • 5. Tran A, Talmud D, Lejeune B, Jovenin N, Renois F, Payan C, et al. Prevalence of rotavirus, adenovirus, norovirus, and astrovirus infections and coinfections among hospitalized children in northern France. J Clin Microbiol. 2010;48(5):1943-6. doi: 10.1128/JCM.02181-09. [PubMed: 20305010]. [PubMed Central: PMC2863921].
  • 6. Payne DC, Staat MA, Edwards KM, Szilagyi PG, Gentsch JR, Stockman LJ, et al. Active, population-based surveillance for severe rotavirus gastroenteritis in children in the United States. Pediatrics. 2008;122(6):1235-43. doi: 10.1542/peds.2007-3378. [PubMed: 19047240].
  • 7. Basu G, Rossouw J, Sebunya TK, Gashe BA, de Beer M, Dewar JB, et al. Prevalence of rotavirus, adenovirus and astrovirus infection in young children with gastroenteritis in Gaborone, Botswana. East Afr Med J. 2003;80(12):652-5. [PubMed: 15018423].
  • 8. Barnes GL, Uren E, Stevens KB, Bishop RF. Etiology of acute gastroenteritis in hospitalized children in Melbourne, Australia, from April 1980 to March 1993. J Clin Microbiol. 1998;36(1):133-8. [PubMed: 9431936]. [PubMed Central: PMC124823].
  • 9. Cruz JR, Caceres P, Cano F, Flores J, Bartlett A, Torun B. Adenovirus types 40 and 41 and rotaviruses associated with diarrhea in children from Guatemala. J Clin Microbiol. 1990;28(8):1780-4. [PubMed: 2168438]. [PubMed Central: PMC268047].
  • 10. Kamel AH, Ali MA, El-Nady HG, Aho S, Pothier P, Belliot G. Evidence of the co-circulation of enteric viruses in sewage and in the population of Greater Cairo. J Appl Microbiol. 2010;108(5):1620-9. doi: 10.1111/j.1365-2672.2009.04562.x. [PubMed: 19840175].
  • 11. Shoeib AR, Hull JJ, Jiang B. Rotavirus G and P types in children with acute diarrhea in Cairo, Egypt, 2011-2012. J Egypt Public Health Assoc. 2015;90(3):121-4. doi: 10.1097/01.EPX.0000470849.84604.13. [PubMed: 26544841].
  • 12. Saudy N, Elshabrawy WO, Megahed A, Foad MF, Mohamed AF. Genotyping and Clinicoepidemiological Characterization of Rotavirus Acute Gastroenteritis in Egyptian Children. Pol J Microbiol. 2017;65(4):433-42. doi: 10.5604/17331331.1227669. [PubMed: 28735327].
  • 13. Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, et al. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol. 1990;28(2):276-82. [PubMed: 2155916]. [PubMed Central: PMC269590].
  • 14. Gentsch JR, Glass RI, Woods P, Gouvea V, Gorziglia M, Flores J, et al. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol. 1992;30(6):1365-73. [PubMed: 1320625]. [PubMed Central: PMC265294].
  • 15. Gouvea V, de Castro L, Timenetsky MC, Greenberg H, Santos N. Rotavirus serotype G5 associated with diarrhea in Brazilian children. J Clin Microbiol. 1994;32(5):1408-9. [PubMed: 8051281]. [PubMed Central: PMC263717].
  • 16. Puig M, Jofre J, Lucena F, Allard A, Wadell G, Girones R. Detection of adenoviruses and enteroviruses in polluted waters by nested PCR amplification. Appl Environ Microbiol. 1994;60(8):2963-70. [PubMed: 8085832]. [PubMed Central: PMC201750].
  • 17. El-Senousy WM, Barakat AB, Ghanem HE, Kamel MA. Molecular epidemiology of human adenoviruses and rotaviruses as candidate viral indicators in the Egyptian sewage and water samples. World Appl Sci J. 2013;27(10):1235-47.
  • 18. Carraturo A, Catalani V, Tega L. Microbiological and epidemiological aspects of rotavirus and enteric adenovirus infections in hospitalized children in Italy. New Microbiol. 2008;31(3):329-36. [PubMed: 18843886].
  • 19. Motamedifar M, Amini E, Talezadeh Shirazi P. Frequency of rotavirus and adenovirus gastroenteritis among children in shiraz, iran. Iran Red Crescent Med J. 2013;15(8):729-33. doi: 10.5812/ircmj.4415. [PubMed: 24578843]. [PubMed Central: PMC3918200].
  • 20. Cicek C, Karatas T, Altuglu I, Koturoglu G, Kurugol Z, Bilgic A. Comparison of ELISA with shell vial cell culture method for the detection of human rotavirus in fecal specimens. New Microbiol. 2007;30(2):113-8. [PubMed: 17619254].
  • 21. Lin HC, Kao CL, Lu CY, Lee CN, Chiu TF, Lee PI, et al. Enteric adenovirus infection in children in Taipei. J Microbiol Immunol Infect. 2000;33(3):176-80. [PubMed: 11045381].
  • 22. Turcios RM, Curns AT, Holman RC, Pandya-Smith I, LaMonte A, Bresee JS, et al. Temporal and geographic trends of rotavirus activity in the United States, 1997-2004. Pediatr Infect Dis J. 2006;25(5):451-4. doi: 10.1097/01.inf.0000214987.67522.78. [PubMed: 16645512].
  • 23. Santos N, Hoshino Y. Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol. 2005;15(1):29-56. doi: 10.1002/rmv.448. [PubMed: 15484186].

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