Archives of Pediatric Infectious Diseases

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Molecular Identification and Genotyping of Toxoplasma Gondii in Blood and CSF Samples of Children with Immunodeficiency in Ahvaz, Southwest of Iran

Marjan Sabzevari 1 , Mehdi Tavalla 1 , 2 , * and Molouk Beiromvand 1
Authors Information
1 Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2 Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Archives of Pediatric Infectious Diseases: January 31, 2019, 7 (1); e79844
  • Published Online: December 9, 2018
  • Article Type: Research Article
  • Received: May 27, 2018
  • Revised: September 1, 2018
  • Accepted: September 15, 2018
  • DOI: 10.5812/pedinfect.79844

To Cite: Sabzevari M, Tavalla M, Beiromvand M. Molecular Identification and Genotyping of Toxoplasma Gondii in Blood and CSF Samples of Children with Immunodeficiency in Ahvaz, Southwest of Iran, Arch Pediatr Infect Dis. 2019 ; 7(1):e79844. doi: 10.5812/pedinfect.79844.

Abstract
Copyright © 2018, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Methods
3. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Bhopale GM. Development of a vaccine for toxoplasmosis: Current status. Microbes Infect. 2003;5(5):457-62. [PubMed: 12738002].
  • 2. Dubey JP. Toxoplasmosis–a waterborne zoonosis. Vet Parasitol. 2004;126(1-2):57-72. doi: 10.1016/j.vetpar.2004.09.005.
  • 3. Macpherson CN. Human behaviour and the epidemiology of parasitic zoonoses. Int J Parasitol. 2005;35(11-12):1319-31. doi: 10.1016/j.ijpara.2005.06.004. [PubMed: 16102769].
  • 4. Velimirovic B. Toxoplasmosis in immunosuppression and AIDS. Infection. 1984;12(5):315-7. [PubMed: 6392101].
  • 5. Ashburn D, Davidson MM, Joss AW, Pennington TH, Ho-Yen DO. Improved diagnosis of reactivated toxoplasmosis. Mol Pathol. 1998;51(2):105-9. [PubMed: 9713595]. [PubMed Central: PMC395619].
  • 6. Garcia JL, Navarro IT, Vidotto O, Gennari SM, Machado RZ, da Luz Pereira AB, et al. Toxoplasma gondii: Comparison of a rhoptry-ELISA with IFAT and MAT for antibody detection in sera of experimentally infected pigs. Exp Parasitol. 2006;113(2):100-5. doi: 10.1016/j.exppara.2005.12.011. [PubMed: 16458299].
  • 7. Meek B, Diepersloot RJ, van Gool T, Speijer D, Peek R. Igm recognition of recombinant Toxoplasma gondii antigens by sera of acutely or latently infected humans. Diagn Microbiol Infect Dis. 2003;45(1):45-52. [PubMed: 12573550].
  • 8. Garcia JL, Gennari SM, Machado RZ, Navarro IT. Toxoplasma gondii: Detection by mouse bioassay, histopathology, and polymerase chain reaction in tissues from experimentally infected pigs. Exp Parasitol. 2006;113(4):267-71. doi: 10.1016/j.exppara.2006.02.001. [PubMed: 16545804].
  • 9. Kong QM, Lu SH, Tong QB, Lou D, Chen R, Zheng B, et al. Loop-mediated isothermal amplification (LAMP): Early detection of Toxoplasma gondii infection in mice. Parasit Vectors. 2012;5:2. doi: 10.1186/1756-3305-5-2. [PubMed: 22214421]. [PubMed Central: PMC3280158].
  • 10. Homan WL, Vercammen M, De Braekeleer J, Verschueren H. Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int J Parasitol. 2000;30(1):69-75. [PubMed: 10675747].
  • 11. Behzadi R, Razavi MR, Hovanessian A, Asmar M, Rouhvand F. Genotyping of Toxoplasma gondii strains isolated from patients and mice by PCR-RFLP assay. Iran J Biotechnol. 2003;1(2):82-6.
  • 12. Howe DK, Honore S, Derouin F, Sibley LD. Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis. J Clin Microbiol. 1997;35(6):1411-4. [PubMed: 9163454]. [PubMed Central: PMC229759].
  • 13. Mondragon R, Howe DK, Dubey JP, Sibley LD. Genotypic analysis of Toxoplasma gondii isolates from pigs. J Parasitol. 1998;84(3):639-41. [PubMed: 9645877].
  • 14. Cristina N, Derouin F, Pelloux H, Pierce R, Cesbron-Delauwn MF, Ambroise-Thomas P. [Detection of Toxoplasma gondii by "polymerase chain reaction" (PCR) technique in AIDS infected patients using the repetitive sequence TGR1E]. Pathol Biol (Paris). 1992;40(1):52-5. French. [PubMed: 1570184].
  • 15. Reischl U, Bretagne S, Kruger D, Ernault P, Costa JM. Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect Dis. 2003;3:7. [PubMed: 12729464]. [PubMed Central: PMC156600].
  • 16. Fallahi S, Kazemi B, Seyyed tabaei SJ, Bandehpour M, Lasjerdi Z, Taghipour N, et al. Comparison of the RE and B1 gene for detection of Toxoplasma gondii infection in children with cancer. Parasitol Int. 2014;63(1):37-41. doi: 10.1016/j.parint.2013.08.005. [PubMed: 23993996].
  • 17. Fuentes I, Rubio JM, Ramirez C, Alvar J. Genotypic characterization of Toxoplasma gondii strains associated with human toxoplasmosis in Spain: Direct analysis from clinical samples. J Clin Microbiol. 2001;39(4):1566-70. doi: 10.1128/JCM.39.4.1566-1570.2001. [PubMed: 11283088]. [PubMed Central: PMC87971].
  • 18. Zia-Ali N, Keshavarz-Valian H, Rezaian M, Khorramizadeh MR, Kazemi B, Fazaeli A, et al. Molecular characterization of Toxoplasma gondii from bird hosts. Iran J Public Health. 2005;34(3):27-30.
  • 19. Zia-Ali N, Fazaeli A, Khoramizadeh M, Ajzenberg D, Darde M, Keshavarz-Valian H. Isolation and molecular characterization of Toxoplasma gondii strains from different hosts in Iran. Parasitol Res. 2007;101(1):111-5. doi: 10.1007/s00436-007-0461-7. [PubMed: 17333278].
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