Archives of Pediatric Infectious Diseases

Published by: Kowsar

Molecular Typing of Klebsiella pneumoniae Isolated from Medical Centers in Kermanshah Using Pulse Field Gel Electrophoresis

Azam Elahi 1 , Alisha Akya 1 , Roya Chegene Lorestani 1 , Keyghobad Ghadiri 1 and Shokofe Baakhshii 2 , *
Authors Information
1 Nosocomial Infections Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Department of Biology, Faculty of Basic Sciences, Islamic Azad University of Boroujerd, Khorramabad, Iran
Article information
  • Archives of Pediatric Infectious Diseases: 7 (2); e84331
  • Published Online: April 14, 2019
  • Article Type: Research Article
  • Received: September 16, 2018
  • Revised: January 22, 2019
  • Accepted: March 2, 2019
  • DOI: 10.5812/pedinfect.84331

To Cite: Elahi A, Akya A, Chegene Lorestani R, Ghadiri K, Baakhshii S. Molecular Typing of Klebsiella pneumoniae Isolated from Medical Centers in Kermanshah Using Pulse Field Gel Electrophoresis, Arch Pediatr Infect Dis. Online ahead of Print ; 7(2):e84331. doi: 10.5812/pedinfect.84331.

Abstract
Copyright © 2019, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Martin RM, Bachman MA. Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol. 2018;8:4. doi: 10.3389/fcimb.2018.00004. [PubMed: 29404282]. [PubMed Central: PMC5786545].
  • 2. Paterson DL, Ko WC, Von Gottberg A, Mohapatra S, Casellas JM, Goossens H, et al. Antibiotic therapy for Klebsiella pneumoniae bacteremia: implications of production of extended-spectrum beta-lactamases. Clin Infect Dis. 2004;39(1):31-7. doi: 10.1086/420816. [PubMed: 15206050].
  • 3. Paczosa MK, Mecsas J. Klebsiella pneumoniae: Going on the offense with a strong defense. Microbiol Mol Biol Rev. 2016;80(3):629-61. doi: 10.1128/MMBR.00078-15. [PubMed: 27307579]. [PubMed Central: PMC4981674].
  • 4. Apondi OE, Oduor OC, Gye BK, Kipkoech MK. High prevalence of multi-drug resistant Klebsiella pneumoniae in a tertiary teaching hospital in Western Kenya. Afr J Infect Dis. 2016;10(2):89-95. doi: 10.21010/ajid.v10i2.3. [PubMed: 28480442]. [PubMed Central: PMC5411993].
  • 5. Benner KW, Prabhakaran P, Lowros AS. Epidemiology of infections due to extended-spectrum Beta-lactamase-producing bacteria in a pediatric intensive care unit. J Pediatr Pharmacol Ther. 2014;19(2):83-90. doi: 10.5863/1551-6776-19.2.83. [PubMed: 25024667]. [PubMed Central: PMC4093669].
  • 6. Azimi L, Erajiyan G, Talebi M, Owlia P, Bina M, Shojaie A, et al. Phenotypic and molecular characterization of plasmid mediated ampc among clinical isolates of Klebsiella pneumoniae isolated from different Hospitals in Tehran. J Clin Diagn Res. 2015;9(4):DC01-3. doi: 10.7860/JCDR/2015/11037.5797. [PubMed: 26046018]. [PubMed Central: PMC4437065].
  • 7. Ali Abdel Rahim KA, Ali Mohamed AM. Prevalence of extended spectrum Beta-lactamase-producing Klebsiella pneumoniae in clinical isolates. Jundishapur J Microbiol. 2014;7(11). e17114. doi: 10.5812/jjm.17114. [PubMed: 25774279]. [PubMed Central: PMC4332241].
  • 8. Johnson AP. Surveillance of antibiotic resistance. Philos Trans R Soc Lond B Biol Sci. 2015;370(1670):20140080. doi: 10.1098/rstb.2014.0080. [PubMed: 25918439]. [PubMed Central: PMC4424431].
  • 9. Akya A, Elahi A, Chegenelorestani R, Rezaee M. Dissemination of multidrug-resistant, class I and II integrons and molecular typing of CTX-M-producing Klebsiella pneumoniae. Int J Appl Basic Med Res. 2018;8(2):100-5. doi: 10.4103/ijabmr.IJABMR_333_16. [PubMed: 29744322]. [PubMed Central: PMC5932916].
  • 10. Li W, Raoult D, Fournier PE. Bacterial strain typing in the genomic era. FEMS Microbiol Rev. 2009;33(5):892-916. doi: 10.1111/j.1574-6976.2009.00182.x. [PubMed: 19453749].
  • 11. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing. J Clin Microbiol. 1995;33(9):2233-9. [PubMed: 7494007]. [PubMed Central: PMC228385].
  • 12. linical and Laboratory Standards Institute CLSI. Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute; 2011.
  • 13. Durmaz R, Otlu B, Koksal F, Hosoglu S, Ozturk R, Ersoy Y, et al. The optimization of a rapid pulsed-field gel electrophoresis protocol for the typing of Acinetobacter baumannii, Escherichia coli and Klebsiella spp. Jpn J Infect Dis. 2009;62(5):372-7. [PubMed: 19762987].
  • 14. Kothari S, Mishra V, Ranjan N, Singh A. Third generation cephalosporin-resistance in Klebsiella pneumoniae isolates: An emerging threat. Int J Basic Clin Pharmacol. 2013;2(1):56. doi: 10.5455/2319-2003.ijbcp20130111.
  • 15. Ashayeri-Panah M, Feizabadi MM, Eftekhar F. Correlation of multi-drug resistance, integron and blaESBL gene carriage with genetic fingerprints of extended-spectrum beta-lactamase producing Klebsiella pneumoniae. Jundishapur J Microbiol. 2014;7(2). e8747. doi: 10.5812/jjm.8747. [PubMed: 25147670]. [PubMed Central: PMC4138679].
  • 16. Ashayeri-Panah M, Eftekhar F, Ghamsari MM, Parvin M, Feizabadi MM. Genetic profiling of Klebsiella pneumoniae: Comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA. Braz J Microbiol. 2013;44(3):823-8. doi: 10.1590/S1517-83822013005000055. [PubMed: 24516423]. [PubMed Central: PMC3910195].
  • 17. Feizabadi MM, Mahamadi-Yeganeh S, Mirsalehian A, Mirafshar SM, Mahboobi M, Nili F, et al. Genetic characterization of ESBL producing strains of Klebsiella pneumoniae from Tehran hospitals. J Infection Develop Countries. 2010;4(10). doi: 10.3855/jidc.1059.
  • 18. Nematzadeh S, Shahcheraghi F, Feizabadi MM, Nikbin VS, Nasehi L. Molecular characterization of CTX-Mbeta-lactamases among Klebsiella pneumoniae isolated from patients at Tehran hospitals. Indian J Med Microbiol. 2011;29(3):254-7. doi: 10.4103/0255-0857.83908. [PubMed: 21860105].
  • 19. Lau YJ, Hu BS, Wu WL, Lin YH, Chang HY, Shi ZY. Identification of a major cluster of Klebsiella pneumoniae isolates from patients with liver abscess in Taiwan. J Clin Microbiol. 2000;38(1):412-4. [PubMed: 10618128]. [PubMed Central: PMC88736].
  • 20. Al-Marzooq F, Mohd Yusof MY, Tay ST. Molecular analysis of ciprofloxacin resistance mechanisms in Malaysian ESBL-producing Klebsiella pneumoniae isolates and development of mismatch amplification mutation assays (MAMA) for rapid detection of gyrA and parC mutations. Biomed Res Int. 2014;2014:601630. doi: 10.1155/2014/601630. [PubMed: 24860827]. [PubMed Central: PMC4000930].
  • 21. Mshana SE, Hain T, Domann E, Lyamuya EF, Chakraborty T, Imirzalioglu C. Predominance of Klebsiella pneumoniae ST14 carrying CTX-M-15 causing neonatal sepsis in Tanzania. BMC Infect Dis. 2013;13:466. doi: 10.1186/1471-2334-13-466. [PubMed: 24099282]. [PubMed Central: PMC3851032].
  • 22. Tijet N, Sheth PM, Lastovetska O, Chung C, Patel SN, Melano RG. Molecular characterization of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae in Ontario, Canada, 2008-2011. PLoS One. 2014;9(12). e116421. doi: 10.1371/journal.pone.0116421. [PubMed: 25549365]. [PubMed Central: PMC4280202].
  • 23. Dedeic-Ljubovic A, Hukic M, Pfeifer Y, Witte W, Padilla E, Lopez-Ramis I, et al. Emergence of CTX-M-15 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in Bosnia and Herzegovina. Clin Microbiol Infect. 2010;16(2):152-6. doi: 10.1111/j.1469-0691.2009.03018.x. [PubMed: 19686282].
  • 24. Christian NA, Roye-Green K, Smikle M. Molecular epidemiology of multidrug resistant extended spectrum beta-lactamase producing Klebsiella pneumoniae at a Jamaican hospital, 2000-2004. BMC Microbiol. 2010;10:27. doi: 10.1186/1471-2180-10-27. [PubMed: 20109209]. [PubMed Central: PMC2824695].
  • 25. Azimi L, Motevallian A, Ebrahimzadeh Namvar A, Asghari B, Lari AR. Nosocomial infections in burned patients in Motahari Hospital, Tehran, Iran. Dermatol Res Pract. 2011;2011:436952. doi: 10.1155/2011/436952. [PubMed: 22203838]. [PubMed Central: PMC3235664].
  • 26. Birren BW, Lai E, Hood L, Simon MI. Pulsed field gel electrophoresis techniques for separating 1- to 50-kilobase DNA fragments. Anal Biochem. 1989;177(2):282-6. doi: 10.1016/0003-2697(89)90052-3. [PubMed: 2729546].
  • 27. Donati V, Feltrin F, Hendriksen RS, Svendsen CA, Cordaro G, Garcia-Fernandez A, et al. Extended-spectrum-beta-lactamases, AmpC beta-lactamases and plasmid mediated quinolone resistance in klebsiella spp. from companion animals in Italy. PLoS One. 2014;9(3). e90564. doi: 10.1371/journal.pone.0090564. [PubMed: 24595207]. [PubMed Central: PMC3942433].
  • 28. Lin CF, Hsu SK, Chen CH, Huang JR, Lo HH. Genotypic detection and molecular epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in a regional hospital in central Taiwan. J Med Microbiol. 2010;59(Pt 6):665-71. doi: 10.1099/jmm.0.015818-0. [PubMed: 20150317].
  • 29. Salimizand H, Shahcheraghi F, Kalantar E, Badmasti F, Mousavi SF. Molecular characterization of class 1 integrons and gene cassettes in multidrug resistant (MDR) Klebsiella spp. isolated from hospitalized and outpatients in Iran, 2009. Iran J Microbiol. 2013;5(1):48-55. [PubMed: 23466743]. [PubMed Central: PMC3577558].
  • 30. Rodriguez EC, Saavedra SY, Leal AL, Alvarez C, Olarte N, Valderrama A, et al. [The spread of KPC-3 Klebsiella pneumoniae in hospitals in Bogota over a three-year period (2008-2010)]. Biomedica. 2014;34 Suppl 1:224-31. Spanish. doi: 10.1590/S0120-41572014000500025. [PubMed: 24968054].

Featured Image:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments